Apparatus and methods for efficient processing of biological samples on slides

• US 7,598,036 B2
• Filed: 06/22/2005
• Issued: 10/06/2009
• Est. Priority Date: 12/23/1998
• Status: Expired due to Fees

• Current Assignee: American Registry LLC
• Original Assignee: American Registry LLC
• Inventors: Chu, Wei-Sing
• Primary Examiner(s): Chunduru; Suryaprabha
• Application Number:
  US11/157,922
• Publication Number:
  US 20050238534A1
• Time in Patent Office: 1,567 Days
• Field of Search: 436/46, 435/6, 435/287, 536/22.1
• US Class Current: 435/6.11
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

Microscope slides which are useful for manually or automatically processing biological samples are described. External controls may be placed directly on the microscope slide in conjunction with a biological sample to be assayed. The external controls can also be conveniently placed on a membrane which can be affixed to the slide.

Claims

1. A slide comprising a microscope slide having a control region apart from a biological sample region and a membrane mounted on the control region of said microscope slide, having at least one control placed on the membrane wherein said membrane comprises said control on a first face of said membrane and a substance on a second face of said membrane wherein said substance causes said membrane to adhere to said microscope slide when contacted with said microscope slide.

2. The slide of claim 1, wherein said substance is glue.

3. The slide of claim 1, wherein said control is selected from the group consisting of:

an external control, a positive control, and a negative control.

4. The slide of claim 1, wherein said control is selected from the group consisting of antigens, peptides, proteins, nucleic acids and cells.

5. The slide of claim 2 wherein said glue is resistant to xylene and alcohol.

6. The slide of claim 1, wherein said membrane is selected from the group consisting of:

nitrocellulose, plastic, glass, and nylon.

7. The slide of claim 1, wherein said membrane is transparent.

8. The slide of claim 1, wherein said membrane comprises a plurality of said controls.

9. A method of assaying a biological sample on said slide of claim 1, comprising one or more of said controls placed on said membrane of said control region and the biological sample placed on said biological sample region and assaying simultaneously said one or more controls and said biological sample.

10. The method of claim 9, wherein said slide is placed on a well of a multiwell tray containing one or more reagents which react with said biological sample.

11. A kit comprising said slide of claim 1 in combination with a multiwell tray.

12. The kit of claim 11, wherein said multiwell tray comprises predried reagents.

13. The kit of claim 11, wherein a reagent added to a well of said multiwell tray reacts with said control on said membrane.