Microarray for predicting the prognosis of neuroblastoma and method for predicting the prognosis of neuroblastoma

• US 7,601,532 B2
• Filed: 09/23/2004
• Issued: 10/13/2009
• Est. Priority Date: 09/25/2003
• Status: Active Grant

• Current Assignee: Chiba-Prefecture, Hisamitsu Pharmaceutical Company Incorporated
• Original Assignee: Chiba-Prefecture, Hisamitsu Pharmaceutical Company Incorporated, NGK Insulators Limited
• Inventors: Goto, Takeshi, Yoshida, Yasuko, Kubo, Hiroyuki, Ohira, Miki, Hirata, Takahiro, Yamada, Saichi, Nakagawara, Akira, Ishii, Shin
• Primary Examiner(s): Bausch; Sarae
• Assistant Examiner(s): SALMON, KATHERINE D
• Application Number:
  US10/947,249
• Publication Number:
  US 20050287541A1
• Time in Patent Office: 1,846 Days
• Field of Search: None
• US Class Current: 435/287.2
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 1/6886 for cancer immunoassay for cancer G01N33/574
• CPC Class Codes: C12Q 2600/118 Prognosis of disease development

Abstract

A microarray for predicting the prognosis of neuroblastoma, wherein the microarray has 25 to 45 probes related to good prognosis, which are hybridized to a gene transcript whose expression is increased in a good prognosis patient with neuroblastoma and are selected from 96 polynucleotides consisting of the nucleotide sequences of SEQ. ID NOs. 1, 5, 6, 14. 16, 17, 19, 22-24, 28, 29, 31, 37, 39, 40, 43, 44, 47-52, 54, 57-60, 62, 64, 65, 67, 68, 72-75, 77, 78, 80-82, 84, 87, 89-91, 94, 100, 103, 112, 113, 118, 120, 129, 130, 132, 136, 138, 142, 144, 145, 148, 150-153, 155, 158-160, 163-165, 169-171, 173, 174, 177, 178, 180-182, 184, 186, 187, 189, 191, 192, 194, 195, 198-200 or their partial continuous sequences or their complementary strands, and 25 to 45 probes related to poor prognosis, which are hybridized to a gene transcript whose expression is increased in a poor prognosis patient with neuroblastoma and are selected from 104 polynucleotides consisting of the nucleotide sequences of SEQ. ID NOs. 2-4, 7-13, 15, 18, 20, 21, 25-27, 30, 32-36, 38, 41, 42, 45, 46, 53, 55, 56, 61, 63, 66, 69-71, 76, 79, 83, 85, 86, 88, 92, 93, 95-99, 101, 102, 104-111, 114-117, 119, 121-128, 131, 133-135, 137, 139-141, 143, 146, 147, 149, 154, 156, 157, 161, 162, 166-168, 172, 175, 176, 179, 183, 185, 188, 190, 193, 196, 197 or their partial continuous sequences or their complementary strands.

Claims

1. A microarray, comprising:

a first combination of 25 different probes respectively comprising the sequences set forth in SEQ ID NOs;

5, 14, 16, 17, 22, 23, 28, 37, 51, 64, 67, 87, 113, 129, 132, 136, 142, 152, 155, 163, 165, 171, 173, and 191, and full-length complementary sequences thereof, and

a second combination of 25 different probes respectively comprising the sequences set forth in SEQ ID NOs;

2, 4, 7, 13, 18, 20, 25, 26, 34, 38, 42, 46, 55, 70, 79, 83, 86, 92, 105, 114, 119, 124, 172, 179, and 183, and full-length complementary sequences thereof.