Antibody peg positional isomers, compositions comprising same, and use thereof

• US 7,601,817 B2
• Filed: 05/28/2003
• Issued: 10/13/2009
• Est. Priority Date: 05/28/2002
• Status: Active Grant

• Current Assignee: UCB SA
• Original Assignee: UCB Pharma SA (UCB SA)
• Inventors: Mozier, Ned M.
• Primary Examiner(s): Szperka; Michael
• Assistant Examiner(s): Kim; Yunsoo
• Application Number:
  US10/515,677
• Publication Number:
  US 20060110382A1
• Time in Patent Office: 2,330 Days
• Field of Search: None
• US Class Current: 530/388.1
• CPC Class Codes: A61K 2039/505 comprising antibodies
• CPC Class Codes: A61K 47/60 the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
• CPC Class Codes: A61P 1/04 for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
• CPC Class Codes: A61P 1/16 for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• CPC Class Codes: A61P 11/00 Drugs for disorders of the respiratory system
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 17/06 Antipsoriatics
• CPC Class Codes: A61P 19/02 for joint disorders, e.g. arthritis, arthrosis
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 31/18 for HIV
• CPC Class Codes: A61P 37/00 Drugs for immunological or allergic disorders
• CPC Class Codes: A61P 37/08 Antiallergic agents antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: C07K 16/00 Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies antibodies with enzymatic activity, e.g. abzymes C12N9/0002
• CPC Class Codes: C07K 16/241 Tumor Necrosis Factors
• CPC Class Codes: C07K 2317/24 containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered

Abstract

The present invention relates to recombinant protein PEG positional isomers. More specifically, it relates to PEG positional isomers of an antibody having specificity for antigenic determinants of human tumor necrosis factor alpha (TNFα). More specifically, it relates to PEG positional isomers of CDP870. The present invention also relates to compositions comprising the PEG positional isomers and therapeutic uses of the composition for treating a disease medicated by TNFα including acute and chronic immune and immunoregulatory disorders and rheumatoid-or osteo-arthritis.

Claims

1. An isolated antibody molecule which is a modified Fab fragment, the amino acid sequences of whose light chain (SEQ ID NO:

1) and heavy chain (SEQ ID NO;

2) are as depicted in FIG. 2, wherein a polyethylene glycol (PEG) moiety is attached at Cys 214 of the light chain.

2. The isolated antibody molecule of claim 1 further comprising a disulfide linkage between Cys 221 and Cys 227 of the heavy chain.

3. A therapeutic or diagnostic composition comprising the isolated antibody molecule of claim 1 in association with a pharmaceutically acceptable carrier.

4. A therapeutic or diagnostic composition comprising the isolated antibody molecule of claim 2 in association with a pharmaceutically acceptable carrier.