High-speed, rugged, time-resolved, raman spectrometer for sensing multiple components of a sample

• US 7,602,488 B2
• Filed: 06/12/2006
• Issued: 10/13/2009
• Est. Priority Date: 06/12/2006
• Status: Expired due to Fees

• Current Assignee: Optical Oncology, Inc.
• Original Assignee: Neptec Optical Solutions, Inc.
• Inventors: Claps, Ricardo, Smith, Bradley A.
• Primary Examiner(s): Geisel; Kara E
• Application Number:
  US11/452,129
• Publication Number:
  US 20070285658A1
• Time in Patent Office: 1,219 Days
• Field of Search: 356/301, 250/227.23, 250/230, 250/232, 250/236
• US Class Current: 356/301
• CPC Class Codes: G01J 2003/123 Indexed discrete filters
• CPC Class Codes: G01J 3/0218 using optical fibers
• CPC Class Codes: G01J 3/0227 using notch filters
• CPC Class Codes: G01J 3/1895 using fiber Bragg gratings or gratings integrated in a waveguide
• CPC Class Codes: G01J 3/26 using multiple reflection, e.g. Fabry-Perot interferometer, variable interference filters
• CPC Class Codes: G01J 3/2889 Rapid scan spectrometers; Time resolved spectrometry
• CPC Class Codes: G01J 3/32 Investigating bands of a spectrum in sequence by a single detector
• CPC Class Codes: G01J 3/44 Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
• CPC Class Codes: G01N 21/65 Raman scattering

Abstract

A new architecture for implementing a time-resolved Raman spectrometer is 2-3 orders of magnitude faster than current systems. The system additionally is compact, environmentally rugged, low cost and can detect multiple components of a sample simultaneously. In one embodiment, the invention employs a rotating optical switch to time multiplex an input signal through multiple bandpass filters and into a single optical detector which is electrically activated only when the filtered input light pulse is about to impact it.

The combination of time-multiplexing the input signal through multiple optical filters and time-sequencing the optical detector enables the device to detect and analyze 2-3 orders of magnitude faster than current designs, processing spectra within milliseconds instead of seconds. The system can process multiple material samples (25+) simultaneously, instead of sequentially, and its mechanical ruggedness and simplicity enables using the system in harsh physical environments when traditional spectrometers can not be used reliably.

Claims

1. A Raman spectroscopy system comprising:

a light source for producing light to be directed at a sample;

a time-division multiplexing device for taking Stokes radiation scattered from the sample as a result of said light impinging on said sample and distributing that Stokes radiation to each of a plurality of filters;

a light transmission channel from each of the filters to a detector;

a synchronizing circuit for turning on said detector at the time that a signal from said multiplexing device is expected at said detector; and

analysis means connected to said detector for processing each signal from said detector to detect selected information in each signal.

2. The system of claim 1 wherein said synchronizing circuit synchronizes the turning on of said detector with the arrival at said detector of a signal from said time-division multiplexing device.

3. The system of claim 1 wherein said synchronizing circuit synchronizes the turning on of said detector with the arrival at said detector of a signal from said time division multiplexing device so as to maximize the sensitivity of the system.

4. The system of claim 1 wherein said synchronizing circuit synchronizes the turning on of said detector with the arrival at said detector of a signal from said time-division multiplexing device so as to reduce the dark count and noise level present at said analysis means.

5. The system of claim 1 wherein said synchronizing circuit synchronizes the turning on of said detector with the arrival at said detector of a signal from said time-division multiplexing device so as to both maximize the sensitivity of the detector and reduce the dark count and the noise level at the analysis means.

6. The system of claim 1 wherein said analysis means is configured to utilize what is known as a discrete principle component analysis procedure to analyze the signals from the detector to determine the nature of one or more analytes in the sample.

7. The system of claim 6 wherein said discrete principle component analysis procedure is specifically configured to enable the analysis means to detect two or more analytes in said sample in one cycle of rotation of the time division multiplexing device.

8. The system of claim 6 wherein said discrete principle component analysis procedure is specifically configured to enable the analysis means to detect two or more discrete multiple analytes in said sample in one cycle of rotation of the time division multiplexing device.

9. The system of claim 1 including:

means for holding said sample such that said light can impinge on said sample.

10. A Raman spectroscopy system comprising:

a time-division multiplexing device for distributing Stokes radiation scattered from a sample as a result of light impinging on said sample to each of a plurality of transmission channels;

a light detector for detecting the Stokes radiation passed by each of said transmission channels

a synchronizing circuit for turning on said detector when Stokes radiation from said multiplexing device is expected at said detector; and

a plurality of filters formed on a one-to-one basis as a part of said plurality of transmission channels.

11. The system of claim 10 further comprising:

analysis means for processing a signal from said detector when said detector is impacted by Stokes radiation to detect selected information in said Stokes radiation.

12. The system of claim 10 further comprising:

a source of said light; and

means for causing said light to impinge on said sample so as to create said Stokes radiation.