Scanner having spatial light modulator

• US 7,602,952 B2
• Filed: 11/16/2005
• Issued: 10/13/2009
• Est. Priority Date: 11/16/2004
• Status: Active Grant

• Current Assignee: Illumina Incorporated
• Original Assignee: Illumina Incorporated
• Inventors: Moon, John A., Kersey, Alan
• Primary Examiner(s): AZARIAN, SEYED H
• Application Number:
  US11/281,937
• Publication Number:
  US 20060139635A1
• Time in Patent Office: 1,427 Days
• Field of Search: 382/100, 382/103, 382/106, 382/108, 382128-134, 382/162, 382/168, 382180-181, 382/189, 382/255, 382193-194, 382260-261, 382/274, 382/276, 382/305, 382/312, 382/318, 382/147, 250/201.3, 250/458.1, 356/317, 356/310, 359/196, 378/6
• US Class Current: 382/128
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/021 using plane or convex mirrors, parallel phase plates, or particular reflectors
• CPC Class Codes: G01J 3/4406 Fluorescence spectrometry
• CPC Class Codes: G01N 2021/6471 Special filters, filter wheel
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G02B 21/0076 arrangements using fluorescence or luminescence

Abstract

The present invention features incorporating an adaptive spectral filter into a confocal scanner optical arrangement or other suitable optical device to permit real time control of the fluorescence signal spectrum being monitored. This new arrangement would allow for better balancing of the fluorescence signals in the analysis of the array.

Claims

1. An optical device or scanner for monitoring fluorescence signals emitted from a surface of a medium, the fluorescent signals varying in intensity across the surface, the optical device or scanner comprising:

an adaptive spectral filter including a spatial light modulator that selectively modulates the fluorescent signals, the spatial light modulator modulating the fluorescent signals emitting from at least one spatial location of the surface based upon an intensity of the fluorescent signals emitting from the corresponding spatial location, wherein the optical device or scanner records an image using multiple fluorescence tags that indicate a degree of binding or hybridization of target biomolecules to probes immobilized on the medium which is used in an assay process, and wherein the adaptive spectral filter is controlled to be spectrally matched to a first fluorescent signal spectrum and is then adjusted to be spectrally matched to a second fluorescent signal spectrum that is different from the first fluorescent signal spectrum.

2. An optical device or scanner according to claim 1, wherein the adaptive spectral filter detects the fluorescence signal spectrum provided from the medium used in an assay process.

3. An optical device or scanner according to claim 1, wherein the adaptive spectral filter has an SLM equalization control module that equalizes intensities of the fluorescence signal spectrum across a predetermined portion of the surface of the spatial light modulator.

4. An optical device or scanner according to claim 1, wherein the spatial light modulator comprises an array of selectively controllable micromirrors.

5. An optical device or scanner according to claim 1, wherein the spatial light modulator comprises at least one of a reflective and a transmissive element.

6. An optical device or scanner according to claim 1, wherein the spatial light modulator is a pixelating device.

7. An optical device or scanner according to claim 1, wherein the optical device or scanner is an imaging biochip/microarray confocal scanner.

8. An optical device or scanner according to claim 1 wherein the medium includes a plurality of microbeads or a microarray.

9. An optical device or scanner according to claim 1 wherein the spatial light modulator attenuates the fluorescent signals associated with the corresponding spatial location when the intensity of the fluorescent signals of the corresponding spatial location exceeds a predetermined value.

10. An optical device or scanner according to claim 1 wherein the surface comprises a first spatial location and a second spatial location adjacent to the first spatial location, the spatial light modulator modulating an intensity of the fluorescent signals emitting from the first spatial location when the intensity of the fluorescent signals from the first spatial location is different from an intensity of the fluorescent signals emitting from the second spatial location.

11. A method for monitoring fluorescence signals emitted from a surface of a medium, the fluorescent signals varying in intensity across the surface, the method comprising:

positioning an adaptive spectral filter to receive the fluorescent signals emitted from the medium, the spectral filter comprising a spatial light modulator; and

selectively modulating the fluorescent signals emitted from the surface of the medium with the spatial light modulator, the fluorescent signals emitted from at least one spatial location of the surface being modulated based upon an intensity of the fluorescent signals emitted from the corresponding spatial location;

recording, at the optical device or scanner, an image using multiple fluorescence tags that indicate a degree of binding or hybridization of target biomolecules to probes immobilized on the medium which is used in an assay process;

controlling the adaptive spectral filter to be spectrally matched to a first fluorescent signal spectrum; and

adjusting the adaptive spectral filter to be spectrally matched to a second fluorescent signal spectrum that is different from the first fluorescent signal spectrum.

12. A method according to claim 11, wherein the method includes detecting the fluorescence signal spectrum provided from the medium used in an assay process with the adaptive spectral filter.

13. A method according to claim 11, wherein the method includes equalizing intensities of the fluorescence signal spectrum across a predetermined portion of the surface of the spatial light modulator with an SLM equalization control module in the adaptive spectral filter.

14. A method according to claim 11, wherein the spatial light modulator comprises an array of selectively controllable micromirrors.

15. A method according to claim 11, wherein the spatial light modulator comprises at least one of a reflective and a transmissive element.

16. A method according to claim 11, wherein the spatial light modulator is a pixelating device.

17. A method according to claim 11, wherein the spatial light modulator is used in an imaging biochip/microarray confocal scanner.

18. A method according to claim 11, further comprising recording an image using multiple fluorescence tags that indicate a degree of binding or hybridization of target biomolecules to probes immobilized on the medium.