Dynamically configurable electrode formed of pixels

• US 7,604,718 B2
• Filed: 02/14/2004
• Issued: 10/20/2009
• Est. Priority Date: 02/19/2003
• Status: Active Grant

• Current Assignee: BioArray Solutions Limited (Werfen SA)
• Original Assignee: BioArray Solutions Limited (Werfen SA)
• Inventors: Zhang, Yi, Seul, Michael
• Primary Examiner(s): Nguyen; Nam X
• Assistant Examiner(s): BALL, JOHN C
• Application Number:
  US10/778,520
• Publication Number:
  US 20040159546A1
• Time in Patent Office: 2,075 Days
• Field of Search: 204/403.01, 204/400, 204/547, 204/643, 204/267, 204/229.4, 204/229.5, 204/600, 204/450, 204/230.6, 204/230.8
• US Class Current: 204/230.6
• CPC Class Codes: B01J 2219/00653 the compounds being bound to electrodes embedded in or on the solid supports
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/089 Virtual walls for guiding liquids
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B03C 5/005 Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength B03C5/02 takes precedence; electrophoresis B01D57/02
• CPC Class Codes: B03C 5/026 using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]

Abstract

A dynamically configurable electrode includes a first planar electrode and a planar array of pixels in a different plane, wherein a polarizable liquid medium (including electrolyte solutions) is to reside in the gap between electrodes. The pixels are individually addressable by a time-varying voltage, and adjacent pixels receive, at any instant in time, either the same voltage waveform or a different voltage waveform. Adjacent pixels receiving different voltage waveforms generate corresponding movement of dipolar entities, including dipolar particles, ions, or dipolar molecules in the polarizable liquid medium between the electrodes, which can in turn generate fluid flow and movement of particles suspended in the fluid along the planar array surface.

Claims

1. A dynamically configurable electrode comprising:

a shielding layer located in a first region;

a planar array of pixels in a second region such that the shielding layer isolates the planar array of pixels from a driving circuit on the side of the shielding layer opposite from the planar array of pixels, said planar array of pixels having an insulating layer thereon, said pixels individually addressable by a time-varying waveform voltage and, adjacent pixels receiving, at any instant in time, either the same voltage waveform or a different voltage waveform such that said planar array of pixels, upon receiving different voltage waveforms, forms a means for initiating recirculation of a polarizable liquid medium and movement of dipolar entities parallel to the planar array and towards one or more regions adjacent said planar array of pixels, wherein the double-layer capacitance at the polarizable liquid medium-pixel interface is smaller than the capacitance of the insulating layer, but is far greater than the stray capacitance between the planar array of pixels and the shielding layer, and

wherein the dipolar entities reside in the polarizable liquid medium and can include dipolar molecules, ions, cells or polarized particles;

wherein said polarizable liquid medium resides adjacent said insulating layer and between a planar electrode, lying parallel to the planar array of pixels and located in a third region, and

wherein the planar electrode provides a reference voltage to the polarizable liquid medium.

2. The electrode of claim 1 wherein, in general, the dipolar entities which are nearer to the interface of the insulator and the polarizable liquid medium move to a greater extent than the dipolar entities which are further from the interface.

3. The electrode of claim 1 wherein the planar array is divided into pixel regions, and contiguous pixels within a region can receive the same voltage waveform at a given time.

4. The electrode of claim 1 wherein the relative magnitude of the waveforms determines the direction of motion of the polarizable medium.

5. The electrode of claim 1 wherein the planar electrode provides a reference voltage to the planar array of pixels of a magnitude such that dipolar entities in the medium are forced adjacent to the planar array surface.

11. The dynamically configurable electrode of claims 1 or 6 wherein the voltage waveforms have a frequency of less than 1 kHz.

6. A programmable dynamically configurable substantially planar electrode for generating recirculation of polarizable liquid medium and movement of dipolar entities, including dipolar molecules, ions, cells and particles, in a polarizable medium, parallel to the planar electrode, comprising:

a shielding layer;

a planar array of pixels in a different plane from the shielding layer such that the shielding layer isolates the planar array of pixels from a driving circuit on the side of the shielding layer opposite from the planar array of pixels, said planar array of pixels having an insulating layer thereon, said pixels individually addressable by a time-varying waveform voltage and, adjacent pixels receiving, at any instant in time, either the same voltage waveform or a different voltage waveform such that said planar array of pixels, upon receiving different voltage waveforms, forms a means for initiating movement of dipolar entities parallel to the planar array and towards one or more regions adjacent said planar array of pixels, wherein the double-layer capacitance at the polarizable liquid medium-pixel interface is smaller than the capacitance of the insulating layer, but is far greater than the stray capacitance between the planar array of pixels and the shielding layer, the polarizable liquid medium being suspended between a second planar electrode, lying parallel to the array of pixels, and the pixels and wherein the second planar electrode provides a reference voltage to the polarizable liquid medium; and

a computer and user interface for controlling the addressing of and the voltage waveforms received by the pixels.

7. The planar electrode of claim 6 wherein the planar array of pixels is divided into pixel regions, and contiguous pixels within a region can receive the same voltage waveform at a given time, and a user can control region contours to form any of a variety of flow patterns on the planar electrode.

8. The planar electrode of claim 6 wherein, in general, the dipolar entities which are nearer to the interface of the insulator and the polarizable liquid medium move to a greater extent than the dipolar entities which are further from the interface.

9. The planar electrode of claim 6 wherein the relative magnitude of the waveforms determines the direction of motion of the polarizable medium.

10. The planar electrode of claim 6 wherein the second planar electrode provides a reference voltage to the planar array of a magnitude such that dipolar entities in the medium are forced adjacent to the planar array surface.