Thermal reaction device and method for using the same
First Claim
Patent Images
1. A method for performing an analysis of a sample comprising the steps of:
- a) providing a microfluidic device comprising;
a sample channel having cross-sectional dimensions between 0.1 and 1000 μ
m,valves positioned such that the sample channel is partitioned into a plurality of fluidically isolated chambers by activation of said valves, said valves being formed in said microfluidic device by one or more deflectable membrane(s) that can be deflected into said sample channel,wherein said deflectable membrane is formed from an elastomeric material having a Young'"'"'s modulus in the range 20 Pa to 1 GPa,wherein said fluidically isolated chambers are not located at junctions formed by the sample channel and other flow channels that are in fluid communication with the sample channel, andwherein said sample channel has an entrance for introduction of a fluid, but does not have a separate exitb) introducing a sample fluid comprising reagents for nucleic acid amplification and at least one amplifiable target into said sample channel,c) simultaneously actuating said valves to produce at least 500 fluidically isolated chambers, wherein at least one amplifiable target is present in at least one chamber,d) conducting amplification steps simultaneously in said at least 500 fluidically isolated chambers wherein an amplified target is produced in at least one chamber, ande) detecting said amplified target.
4 Assignments
0 Petitions
Accused Products
Abstract
An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
-
Citations
21 Claims
-
1. A method for performing an analysis of a sample comprising the steps of:
-
a) providing a microfluidic device comprising; a sample channel having cross-sectional dimensions between 0.1 and 1000 μ
m,valves positioned such that the sample channel is partitioned into a plurality of fluidically isolated chambers by activation of said valves, said valves being formed in said microfluidic device by one or more deflectable membrane(s) that can be deflected into said sample channel, wherein said deflectable membrane is formed from an elastomeric material having a Young'"'"'s modulus in the range 20 Pa to 1 GPa, wherein said fluidically isolated chambers are not located at junctions formed by the sample channel and other flow channels that are in fluid communication with the sample channel, and wherein said sample channel has an entrance for introduction of a fluid, but does not have a separate exit b) introducing a sample fluid comprising reagents for nucleic acid amplification and at least one amplifiable target into said sample channel, c) simultaneously actuating said valves to produce at least 500 fluidically isolated chambers, wherein at least one amplifiable target is present in at least one chamber, d) conducting amplification steps simultaneously in said at least 500 fluidically isolated chambers wherein an amplified target is produced in at least one chamber, and e) detecting said amplified target. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
-
Specification