Method of generating islet beta-cells from exocrine pancreatic cells
First Claim
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1. An In vitro method of generating insulin producing beta cells from a population comprising dedifferentiated exocrine pancreatic cells of a first mammal, said method comprising the steps of:
- a) providing a population comprising dedifferentiated exocrine pancreatic cells in a culture medium,b) adding one or more ligands of the gp130 receptor of a second mammal and adding one or more ligands of the EGF receptor of a third mammal to said culture medium,c) incubating said dedifferentiated exocrine pancreatic cells in said culture medium comprising said one or more ligands of the gp130 receptor and said one or more ligands of the EGF receptor,wherein one of said one or more ligands of the EGF receptor is EGF.
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Abstract
The present invention discloses an in vitro method wherein mammalian beta-cell differentiation can be induced in dedifferentiated exocrine pancreatic cells in a medium comprising ligands of the EGF receptor and the GP130 receptor, such as EGF and LIF. Insulin secreting cells, obtainable by this method, provide a means for the treatment of diabetes by islet transplantation.
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Citations
12 Claims
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1. An In vitro method of generating insulin producing beta cells from a population comprising dedifferentiated exocrine pancreatic cells of a first mammal, said method comprising the steps of:
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a) providing a population comprising dedifferentiated exocrine pancreatic cells in a culture medium, b) adding one or more ligands of the gp130 receptor of a second mammal and adding one or more ligands of the EGF receptor of a third mammal to said culture medium, c) incubating said dedifferentiated exocrine pancreatic cells in said culture medium comprising said one or more ligands of the gp130 receptor and said one or more ligands of the EGF receptor, wherein one of said one or more ligands of the EGF receptor is EGF. - View Dependent Claims (2, 3, 4, 5, 6, 12)
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7. A population of mammalian pancreatic cells comprising mammalian insulin producing beta cells obtainable by an in vitro method of generating insulin producing beta cells from a population comprising dedifferentiated exocrine pancreatic cells of a first mammal, said method comprising the steps of:
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a) providing a population comprising dedifferentiated exocrine pancreatic cells in a culture medium, b) adding one or more ligands of the gp130 receptor of a second mammal and adding one or more ligands of the EGF receptor of a third mammal to said culture medium, c) incubating said dedifferentiated exocrine pancreatic cells in said culture medium comprising said one or more ligands of the gp130 receptor and said one or more ligands of the EGF receptor, wherein one of said one or more ligands of the EGF receptor is EGF. - View Dependent Claims (8, 9, 10, 11)
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Specification