Compositions and methods for preparing oligonucleotide solutions
First Claim
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1. A method for multiplex detection of target nucleic acids comprising:
- a) providing a first substrate comprising greater than 50 different oligonucleotides linked to said first substrate through cleavable linkers, said greater than 50 different oligonucleotides having sequences different from each other, wherein said substrate comprises an array of discrete sites to which said greater than 50 different oligonucleotides are covalently linked;
b) cleaving said linkers, thereby releasing said greater than 50 different oligonucleotides from said substrate thereby generating a pool of oligonucleotides comprising said greater than 50 different oligonucleotides;
c) contacting said pool of oligonucleotides with a composition comprising different target nucleic acids, whereby said different target nucleic acids hybridize with said greater than 50 different oligonucleotides in said pool of oligonucleotides;
d) modifying said greater than 50 different oligonucleotides in said pool of oligonucleotides hybridized with said different target nucleic acids to produce modified oligonucleotides, ande) contacting said modified oligonucleotides with a second substrate comprising probe oligonucleotides, said probe oligonucleotides having sequences different from each other and having sequences different from said greater than 50 different oligonucleotides released from said first substrate, whereby said target nucleic acids are detected.
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Abstract
The present invention is directed to methods and compositions for generating a pool of oligonucleotides. The invention finds use in preparing a population or subpopulations of oligonucleotides in solution. The pool of oligonucleotides finds use in a variety of nucleic acid detection and/or amplification assays.
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Citations
22 Claims
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1. A method for multiplex detection of target nucleic acids comprising:
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a) providing a first substrate comprising greater than 50 different oligonucleotides linked to said first substrate through cleavable linkers, said greater than 50 different oligonucleotides having sequences different from each other, wherein said substrate comprises an array of discrete sites to which said greater than 50 different oligonucleotides are covalently linked; b) cleaving said linkers, thereby releasing said greater than 50 different oligonucleotides from said substrate thereby generating a pool of oligonucleotides comprising said greater than 50 different oligonucleotides; c) contacting said pool of oligonucleotides with a composition comprising different target nucleic acids, whereby said different target nucleic acids hybridize with said greater than 50 different oligonucleotides in said pool of oligonucleotides; d) modifying said greater than 50 different oligonucleotides in said pool of oligonucleotides hybridized with said different target nucleic acids to produce modified oligonucleotides, and e) contacting said modified oligonucleotides with a second substrate comprising probe oligonucleotides, said probe oligonucleotides having sequences different from each other and having sequences different from said greater than 50 different oligonucleotides released from said first substrate, whereby said target nucleic acids are detected. - View Dependent Claims (6, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19, 20, 21, 22)
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2. A method for multiplex detection of target nucleic acids comprising:
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a) providing a first substrate comprising greater than 50 different oligonucleotides covalently linked to said first substrate through cleavable linkers, said greater than 50 different oligonucleotides having sequences different from each other, wherein said first substrate comprises an array of discrete sites to which said great than 50 different oligonucleotides are covalently linked; b) cleaving said linkers, thereby releasing said greater than 50 different oligonucleotides from said first substrate thereby generating a pool of oligonucleotides comprising said greater than 50 different oligonucleotides; c) contacting said pool of oligonucleotides with different target nucleic acids; d) modifying said greater than 50 different oligonucleotides in said pool of oligonucleotides contacted with said different target nucleic acids to produce modified oligonucleotides; e) contacting said modified oligonucleotides with a second substrate comprising probe oligonucleotides, said probe oligonucleotides having sequences different from each other and having sequences different from said pool of oligonucleotides released from said substrate, and f) detecting said target nucleic acids.
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3. A method for multiplex detection of target nucleic acids comprising:
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a) cleaving greater than 50 different oligonucleotides linked to a first substrate through at least a first cleavable linker from said first substrate, wherein said first substrate comprises an array of discrete sites to which said greater than 50 different oligonucleotides are covalently linked, thereby releasing said greater than 50 different oligonucleotides from said first substrate generating a pool of oligonucleotides, said greater than 50 different oligonucleotides having sequences different from each other; b) contacting said pool of oligonucleotides with different target nucleic acids; c) modifying said greater than 50 different oligonucleotides contacted with said different target nucleic acids to produce modified oligonucleotides, and d) contacting said modified oligonucleotides with a second substrate comprising probe oligonucleotides, said probe oligonucleotides having sequences different from each other and having sequences different from said greater than 50 different oligonucleotides cleaved from said first substrate, and d) detecting said target nucleic acids.
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4. A method for multiplex detection of target nucleic acids comprising:
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a) providing a first substrate comprising greater than 50 different oligonucleotides linked to said first substrate through cleavable linkers, said greater than 50 different oligonucleotides having different sequences, wherein said first substrate comprises an array of discrete sites to which said greater than 50 different oligonucleotides are covalently linked; b) cleaving said linkers, thereby releasing said greater than 50 different oligonucleotides from said substrate thereby generating a pool of oligonucleotides comprising said greater than 50 different oligonucleotides; c) contacting said pool of oligonucleotides with a composition comprising different target nucleic acids, whereby said target nucleic acids hybridize with said greater than 50 different oligonucleotides in said pool of oligonucleotides; d) modifying said greater than 50 different oligonucleotides hybridized with said target nucleic acids to produce modified oligonucleotides; and e) contacting said modified oligonucleotides with a second substrate comprising probe oligonucleotides, said probe oligonucleotides having sequences being different from each other and having sequences different from said greater than 50 different oligonucleotides released from said first substrate, said probe oligonucleotides being distributed randomly on said second substrate, whereby said target nucleic acids are detected.
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5. A method for multiplex detection of target nucleic acids comprising:
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a) providing a first substrate on which are disposed different beads having greater than 50 different oligonucleotides covalently linked to said different beads through cleavable linkers, said greater than 50 different oligonucleotides having sequences different from each other, wherein said first substrate comprises an array of discrete sites on which said beads are disposed; b) cleaving said linkers, thereby releasing said greater than 50 different oligonucleotides from said beads, thereby generating a pool of oligonucleotides comprising said greater than 50 different oligonucleotides; c) contacting said pool of oligonucleotides with different target nucleic acids; d) modifying said greater than 50 different oligonucleotides in said pool of oligonucleotides contacted with said different target nucleic acids to produce modified oligonucleotides; e) contacting said modified oligonucleotides with a second substrate comprising probe oligonucleotides, said probe oligonucleotides having sequences different from each other and having sequences different from said pool of oligonucleotides cleaved from said beads, and f) detecting said target nucleic acids. - View Dependent Claims (15)
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Specification