Compounds and methods for labeling oligonucleotides
First Claim
1. A composition comprising a labeled oligonucleotide comprising at least two different reporter moieties linked to different nucleotides of the oligonucleotide, wherein one of the reporter moieties is a quencher linked through an oxime bond.
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Abstract
The invention provides a novel method of labeling oligonucleotides, with reporter moieties, including but not limited to, quenchers, fluorophores, biotin, digoxigenin, peptides and proteins. In addition, this invention provides a method of detecting hybridization of oligonucleotides. This invention also provides novel azo quenchers having the general formula shown below.
The invention further provides compositions comprising labeled oligonucleotides and solid supports. The invention also provides kits comprising at least one composition of the present invention.
103 Citations
5 Claims
- 1. A composition comprising a labeled oligonucleotide comprising at least two different reporter moieties linked to different nucleotides of the oligonucleotide, wherein one of the reporter moieties is a quencher linked through an oxime bond.
Specification