Methods, kits and compositions pertaining to combination oligomers and libraries for their preparation

• US 7,605,253 B2
• Filed: 12/26/2006
• Issued: 10/20/2009
• Est. Priority Date: 03/09/2001
• Status: Expired due to Fees

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Boston Probes, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Coull, James M., Hyldig-Nielsen, Jens J., Fiandaca, Mark J., Kristjanson, Mark D., Creasey, Theresa S.
• Primary Examiner(s): Riley; Jezia
• Application Number:
  US11/616,080
• Publication Number:
  US 20070105145A1
• Time in Patent Office: 1,029 Days
• Field of Search: 435/6, 536/23.1, 536/25.3
• US Class Current: 536/25.3
• CPC Class Codes: C07B 2200/11 Compounds covalently bound to a solid support
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2525/107 incorporating a peptide nucleic acid
• CPC Class Codes: C12Q 2531/113 PCR
• CPC Class Codes: C12Q 2565/137 Chromatographic separation
• CPC Class Codes: C40B 20/04 Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
• CPC Class Codes: C40B 30/04 by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
• CPC Class Codes: C40B 40/00 Libraries per se, e.g. arrays, mixtures
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: Y10S 435/81 Packaged device or kit

Abstract

This invention pertains to the field of combination oligomers, including the block synthesis of combination oligomers in the absence of a template, as well as related methods, kits, libraries and other compositions.

Claims

1. A method for forming a terminal oligomer block and a condensing oligomer block from a bifunctional single set library, said method comprising:

(a) providing a bifunctional single set library comprising at least two oligomer blocks;

(b) treating an oligomer block of the bifunctional single set library to thereby remove one or more protecting groups and produce a terminal oligomer block; and

(c) treating an oligomer block of the bifunctional single set library to remove different one or more protecting groups as compared with step (b) and thereby produce a condensing oligomer block.

2. The method of claim 1 further comprising condensing the terminal oligomer block and the condensing oligomer block.

3. The method of claim 2, wherein condensation of the terminal oligomer block with the condensing oligomer block produces a combination oligomer comprising a linker of at least three atoms in length.

4. The method of claim 1, wherein the oligomer blocks of the bifunctional single set library are peptide nucleic acid oligomer, PNA chimera or PNA combination oligomer.