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Amperometric sensor

  • US 7,608,180 B2
  • Filed: 12/21/2005
  • Issued: 10/27/2009
  • Est. Priority Date: 06/29/1999
  • Status: Expired due to Fees
First Claim
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1. A method for determining the concentration of H2O2 in a sample containing H2O2, which method comprises:

  • providing a sensor comprising 2 or 3 electrodes, one of the electrodes being coated with a ferricyanide compound which in reduced form functions as a mediator selective for hydrogen peroxide, wherein the ferricyanide compound has a low solubility in an aqueous phase,applying a potential of about −

    400 mV to the sensor, such that the oxidised form of the mediator is reduced to the reduced form,contacting the sensor with the sample, such that the H2O2 oxidises the reduced form of the mediator to the oxidised form,maintaining the applied potential of about −

    400 mV such that the oxidised form of the mediator generating a current,measuring the generated current.

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