Method for treatment of tendinosis using platelet rich plasma

• US 7,608,258 B2
• Filed: 03/22/2005
• Issued: 10/27/2009
• Est. Priority Date: 04/13/2002
• Status: Active Grant

• Current Assignee: FUJIFILM Healthcare Corp. (Fujifilm Holdings Corporation)
• Original Assignee: Allan Mishra
• Inventors: Mishra, Allan
• Primary Examiner(s): Saucier; Sandra E
• Application Number:
  US11/085,987
• Publication Number:
  US 20050186193A1
• Time in Patent Office: 1,680 Days
• Field of Search: None
• US Class Current: 424/93.72
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 35/16 Blood plasma; Blood serum umbilical cord blood A61K35/51
• CPC Class Codes: A61K 38/1841 Transforming growth factor [TGF]
• CPC Class Codes: A61K 38/1858 Platelet-derived growth factor [PDGF]
• CPC Class Codes: A61K 38/30 Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2 insulin-like growth factor binding protein A61K38/1754
• CPC Class Codes: A61K 38/363 Fibrinogen
• CPC Class Codes: A61K 38/39 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
• CPC Class Codes: A61K 38/4833 Thrombin (3.4.21.5)
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system

Abstract

Methods and kits are disclosed to promote vascularization of tissue in mammals using an autologous platelet-rich plasma composition. The kit includes components for the preparation of platelet-rich plasma from the patient'"'"'s blood and components for administration to an area in need of vascularization. The methods and kits are useful for the treatment of tendinosis, and in particular, lateral epicondylitis and plantar fasciitis.

Claims

1. A method of vascularizing an area of tissue injury in a mammal comprising:

drawing autologous blood from the mammal;

adding an anticoagulant to the autologous blood;

fractioning the autologous blood in a disposable separating tube using a centrifugation device to isolate a platelet-rich plasma composition;

titrating the platelet-rich plasma composition to obtain a pH of about 6.5 to 8.0 creating a buffered platelet-rich plasma; and

administering the buffered platelet-rich plasma to the area of tissue injury of the mammal, whereby platelet growth factors are released from the buffered platelet-rich plasma,

wherein no exogenous activator is administered to the mammal or added to the platelet-rich plasma composition prior to its introduction to tissue with a compromised blood supply, and wherein the tissue with has a compromised blood supply comprises a tendinosis.

2. The method of claim 1, further comprising introducing a composition comprising a local anesthetic agent into the tissue before administering the buffered platelet-rich plasma composition.

3. The method of claim 1, wherein the mammal is selected from the group consisting of horses, cows, sheep, pigs, dogs and cats.

4. The method of claim 1, wherein the mammal is a human.

5. The method of claim 1, wherein the tendinosis is tennis elbow.

6. The method of claim 1, wherein the tendinosis is plantar fasciitis.

7. The method of claim 6, further comprising identifying an area to be treated using ultrasound.

8. The method of claim 7, wherein the buffered platelet-rich plasma composition is administered to a medial and/or central band of the identified fascia.

9. The method of claim 1, wherein the tendinosis is rotator cuff injury.

10. The method of claim 1, further comprising performing physical therapy to promote vascularization of the tendinosis.

11. A method of treating a tendinosis in a mammal consisting of:

determining a site of tendinosis in the mammal;

administering a composition consisting of an anesthetic selected from the group consisting of anbesol, benzocaine, lidocaine, procaine, and bupivicaine, to the tendinosis site; and

administering a platelet-rich plasma composition without activator to the tendinosis site in the mammal.

12. The method of claim 11, further comprising titrating the platelet-rich plasma composition to obtain a pH of about 6.5 to 8.0.