Cell suspension rotating fluidic pump

• US 7,610,942 B2
• Filed: 01/15/2004
• Issued: 11/03/2009
• Est. Priority Date: 01/15/2003
• Status: Expired due to Fees

• Current Assignee: Cytek Biosciences Inc
• Original Assignee: Amnis Corporation (DiaSorin S.p.A.)
• Inventors: Crawford, Michael A., Harui, Norio, Esposito, Richard J., Ortyn, William E.
• Primary Examiner(s): MAUST, TIMOTHY LEWIS
• Application Number:
  US10/757,971
• Publication Number:
  US 20040220472A1
• Time in Patent Office: 2,119 Days
• Field of Search: 141/1, 141/2, 141/11, 141 25- 27, 141/69, 141/271, 366/200, 366/213, 604/181, 436/43
• US Class Current: 141/11
• CPC Class Codes: F04B 13/00 Pumps specially modified to deliver fixed or variable measured quantities
• CPC Class Codes: F04B 9/02 the means being mechanical
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 2015/1413 Hydrodynamic focussing
• CPC Class Codes: G01N 35/1095 for supplying the samples to flow-through analysers for a specific analyser see relevant groups, e.g. under G01N15/00, G01N21/00, G01N27/00, G01N30/00, H01J49/00
• CPC Class Codes: Y10T 436/11 Automated chemical analysis

Abstract

A low pulsatility syringe pump including a duplex bearing set rotatingly supporting a lead screw, and a transmission having a first drive train configured to increase a number of motor rotations required for a single rotation of the lead screw, and a second drive train configured to reduce the number of motor rotations required for a single rotation of the lead screw as compared to the first drive train. Another embodiment also includes a motor configured to rotate the syringe about its own axis, independent of the motion of the lead screw. In this other embodiment, the fluid in the syringe barrel includes objects (such as cells, latex beads, etc.) entrained in the fluid. The rate of rotation (e.g., about three revolutions per second) is chosen such that each object traces a substantially circular pathway in the syringe barrel and remains in suspension.

Claims

1. A method for dispensing a fluid containing substantially uniformly distributed particulates entrained therein, comprising the steps of:

(a) providing a container having a longitudinal axis, the container including a volume of fluid in which is entrained a plurality of particulates;

(b) rotating the container in which the fluid is disposed about its longitudinal axis using a rate of rotation that results in the fluid, the particulates in the fluid, and the container achieving solid body rotation, wherein the container is rotated using a first prime mover; and

(c) dispensing the fluid independently of rotating the container, such that rotation of the container is not required in order for the fluid to be dispensed, wherein the fluid is dispensed using a second prime mover that introduces a member into the container to displace the fluid.

2. A method for dispensing a fluid containing substantially uniformly distributed particulates entrained therein, comprising the steps of:

(a) providing a container having a longitudinal axis, the container including a volume of fluid in which is entrained a plurality of particulates;

(b) rotating the container in which the fluid is disposed about its longitudinal axis using a first prime mover and a rate of rotation that results in the particulates in the fluid tracing a substantially circular pathway; and

(c) dispensing the fluid independently of rotating the container using a second prime mover to manipulate a member introduced into the container to displace the fluid, such that dispensing occurs without requiring the container to be rotating.

3. A method for dispensing a fluid containing substantially uniformly distributed particulates entrained therein, comprising the steps of:

(a) providing a container having a longitudinal axis, the container including a volume of fluid in which is entrained a plurality of particles, the container including a member configured to dispense fluid from the container when the member is manipulated;

(b) rotating the container in which the fluid is disposed about its longitudinal axis before dispensing the fluid in the container using a first prime mover, such that the particulates become substantially uniformly distributed within the fluid in the container;

(c) halting the rotation of the container;

(d) dispensing the fluid when the container is not rotating using a second prime mover to manipulate the member to displace the fluid; and

(e) repeating steps (b), (c), and (d).

4. The method of claim 3, wherein the container is rotated at a rate that results in a solid body rotation of the container, the fluid and the particulates entrained within the fluid.

5. The method of claim 3, wherein the container is rotated at a rate that results in particulates entrained within the fluid tracing a substantially circular path.

6. The method of claim 3, wherein the container is rotated at a rate that is between about one revolution per minute and about ten revolutions per minute.

7. The method of claim 3, wherein the container is rotated at a rate of about three revolutions per minute.

8. The method of claim 3, further comprising the step of ceasing dispensing of the fluid from the container before repeating steps (b), (c), and (d).

9. The method of claim 3, wherein the first prime mover used to rotate the container is a motor, and further comprising the step of matching a frequency modulation and phase characteristics of the motor to a rate of rotation of the container, thereby reducing a pulsatility induced in the dispensing of the fluid.

10. The method of claim 3, further comprising the steps of:

(a) rotating the container about its axis during the step of dispensing the fluid, for a period of time sufficient to enable the particulates to become substantially uniformly distributed within the fluid in the container; and

(b) halting the rotation of the container.

11. A method for dispensing a fluid containing substantially uniformly distributed particulates entrained therein with reduced pulsatility, comprising the steps of:

(a) providing a container having a longitudinal axis, the container including a volume of fluid in which is entrained a plurality of particles, the container including a member configured to dispense fluid from the container when the member is manipulated;

(b) rotating the container in which the fluid is disposed about its longitudinal axis before dispensing the fluid in the container, such that the particulates become substantially uniformly distributed within the fluid in the container, using a first prime mover whose frequency modulation and phase characteristics are matched to a rate at which the container is rotated, to reduce a pulsatility induced in the fluid;

(c) halting the rotation of the container;

(d) dispensing the fluid when the container is not rotating; and

(e) repeating steps (b), (c), and (d).