Device for the carrying out of chemical or biological reactions

• US 7,611,674 B2
• Filed: 01/11/2007
• Issued: 11/03/2009
• Est. Priority Date: 10/01/1999
• Status: Expired due to Fees

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Inventors: Hermann, Thomas, Schurf, Markus, Wagner, Tilmann, Heimberg, Wolfgang, Knulle, Matthias
• Primary Examiner(s): Warden; Jill
• Assistant Examiner(s): LEVKOVICH, NATALIA A
• Application Number:
  US11/651,985
• Publication Number:
  US 20070140926A1
• Time in Patent Office: 1,027 Days
• Field of Search: 422/109, 422/67, 422/116, 422/104, 422/102, 436/50, 436/55
• US Class Current: 422/417
• CPC Class Codes: B01L 2200/147 Employing temperature sensors
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 7/54 using spatial temperature gradients

Abstract

The invention relates to a device for the carrying out of chemical or biological reactions with a reaction vessel receiving element for receiving a microtiter plate with several reaction vessels, wherein the reaction vessel receiving element has several recesses arranged in a regular pattern to receive the respective reaction vessels, a heating device for heating the reaction vessel receiving element, and a cooling device for cooling the reaction vessel receiving element.

The invention is characterised by the fact that the reaction vessel receiving element is divided into several segments. The individual segments are thermally decoupled from one another, and each segment is assigned a heating device which may be actuated independently of the others.

By means of the segmentation of the reaction vessel receiving element, it is possible for zones to be set and held at different temperatures. Since the reaction vessel receiving element is suitable for receiving standard microtiter plates, the device according to the invention may be integrated in existing process sequences.

Claims

1. A thermocycler for processing biological or chemical samples, comprising:

a block comprising physically discrete segments, the segmented block configured to receive a plurality of sample volumes in a standard microtiter plate;

a thermoelectric device comprising a plurality of thermoelectric elements, each thermoelectric element in dedicated thermal communication with only one segment of the block such that each segment is aligned with a thermoelectric device; and

a plurality of temperature equalization plates, each temperature equalization plate being dedicated to provide a substantially uniform temperature to only one segment of the block during thermal cycling such that each segment is aligned with a respective temperature equalization plate, and each temperature equalization plate being configured to circulate a cooling medium therein.

2. The thermocycler of claim 1, wherein each temperature equalization plate comprises a material having a relatively high thermal conductivity with respect to the block.

3. The thermocycler of claim 2, wherein the temperature equalization plate comprises copper.

4. The thermocycler of claim 1, wherein the block comprises metal.

5. The thermocycler of claim 4, wherein the block comprises aluminum.

6. The thermocycler of claim 1, further comprising a cooling element in thermal communication with the thermoelectric elements.

7. The thermocycler of claim 6, wherein the thermoelectric device is disposed between the temperature equalization plate and the cooling element.

8. The thermocycler of claim 1, further comprising a cover having a plurality of heating elements, each heating element corresponding to a respective segment of the block.