Method for improving heat stability of composition containing water-soluble coenzyme-bound glucose dehydrogenase (GDH)

• US 7,611,859 B2
• Filed: 03/29/2006
• Issued: 11/03/2009
• Est. Priority Date: 11/10/2005
• Status: Active Grant

• Current Assignee: Toyoda Gosei Company Limited (Toyota Motor Corporation)
• Original Assignee: Toyoda Gosei Company Limited (Toyota Motor Corporation)
• Inventors: Kitabayashi, Masao
• Primary Examiner(s): Monshipouri; Maryam
• Application Number:
  US11/392,000
• Publication Number:
  US 20070105175A1
• Time in Patent Office: 1,315 Days
• Field of Search: 435/14, 600/316
• US Class Current: 435/14
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1486 using enzyme electrodes, e.g. with immobilised oxidase
• CPC Class Codes: C12N 9/0006 acting on CH-OH groups as donors (1.1)
• CPC Class Codes: C12N 9/96 Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: C12Q 1/32 involving dehydrogenase
• CPC Class Codes: C12Q 1/54 involving glucose or galactose

Abstract

The invention relates to a method for improving the heat stability of glucose dehydrogenase (GDH), comprising a step of combining, in a composition comprising soluble coenzyme-bound glucose dehydrogenase (GDH), the enzyme with any one or more selected from the group consisting of sugar alcohols, carboxyl group-containing compounds, alkali metal-containing compounds, alkali earth metal compounds, ammonium salts, sulfate salts and proteins, thereby improving the heat stability of GDH over that achieved without the inclusion of the compounds.

Claims

1. A method for preparing a glucose sensor comprising (a) combining bovine serum albumin in a composition comprising soluble flavin-bound glucose dehydrogenase (GDH), thereby improving the heat stability of GDH over that achieved without the inclusion of the bovine serum albumin, and (b) heat drying the composition onto the surface of the glucose sensor.

2. The method according to claim 1, wherein the concentration of bovine serum albumin is 0.1% by weight or more after combining bovine serum albumin with the composition comprising soluble flavin-bound glucose dehydrogenase and before heat drying the composition.

3. A glucose sensor prepared by the method of claim 1.

4. A method of measuring glucose concentration using the glucose sensor of claim 3.

5. A glucose sensor prepared by the method of claim 2.

6. A method of measuring glucose concentration using the glucose sensor of claim 5.