×

Method for noncovalently immobilizing a biomolecule on a solid substrate and microarray produced according to the method

  • US 7,611,889 B2
  • Filed: 02/07/2005
  • Issued: 11/03/2009
  • Est. Priority Date: 02/16/2004
  • Status: Expired due to Fees
First Claim
Patent Images

1. A method of producing a microarray, comprising:

  • providing a nonporous solid substrate having an amino functional group attached thereto;

    dissolving a nucleic acid functionalized with an amino group in a solution comprising polyethylene glycol (PEG) to form a solution containing the nucleic acid and the PEGS; and

    transferring the solution containing the nucleic acid and the PEG to the substrate, in order to noncovalently immobilize the nucleic acid on the substrate via noncovalent intervention of the PEG;

    wherein the nonporous solid substrate having an amino functional group attached thereto is a solid substrate with a surface coated with γ

    -aminopropyltriethoxysilane (GAPS), andthe nucleic acid functionalized with an amino group is DNA functionalized with an aminohexyl group at its 5′

    end, andwherein a concentration of the PEG is in a range of 0.4 to 8.0 mM.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×