Method for noncovalently immobilizing a biomolecule on a solid substrate and microarray produced according to the method

• US 7,611,889 B2
• Filed: 02/07/2005
• Issued: 11/03/2009
• Est. Priority Date: 02/16/2004
• Status: Expired due to Fees

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: Huh, Nam, Park, Jong-myeon
• Primary Examiner(s): Haq; Shafiqul
• Application Number:
  US11/052,345
• Publication Number:
  US 20050181407A1
• Time in Patent Office: 1,730 Days
• Field of Search: 435/6, 435/287.2, 435/7.92, 530/402, 530/405
• US Class Current: 435/287.2
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

Provided is a method for noncovalently immobilizing a biomolecule on a solid substrate, including: providing a solid substrate having a first functional group attached thereto, the first functional group having a hydrogen bond donating ability; and reacting a mixture of a compound having a hydrogen bond accepting ability and a biomolecule functionalized with a second functional group, with the surface of the substrate, the second functional group having a hydrogen bond donating ability, in order to noncovalently immobilize the biomolecule on the substrate.

Claims

1. A method of producing a microarray, comprising:

providing a nonporous solid substrate having an amino functional group attached thereto;

dissolving a nucleic acid functionalized with an amino group in a solution comprising polyethylene glycol (PEG) to form a solution containing the nucleic acid and the PEGS; and

transferring the solution containing the nucleic acid and the PEG to the substrate, in order to noncovalently immobilize the nucleic acid on the substrate via noncovalent intervention of the PEG;

wherein the nonporous solid substrate having an amino functional group attached thereto is a solid substrate with a surface coated with γ

-aminopropyltriethoxysilane (GAPS), and

the nucleic acid functionalized with an amino group is DNA functionalized with an aminohexyl group at its 5′

end, and

wherein a concentration of the PEG is in a range of 0.4 to 8.0 mM.

2. The method of claim 1, wherein the PEG has a molecular weight of 200 to 1,000,000 Da.

3. The method of claim 1, wherein a concentration of the nucleic acid is in a range of 20 to 100 μ

M.

4. A microarray produced according to the method of claim 1.