Electroprocessed collagen and tissue engineering

• US 7,615,373 B2
• Filed: 05/28/2003
• Issued: 11/10/2009
• Est. Priority Date: 02/25/1999
• Status: Expired due to Fees

• Current Assignee: Organogenesis Inc. (Organogenesis Holdings, Inc.), Virginia Commonwealth University
• Original Assignee: Organogenesis Inc. (Organogenesis Holdings, Inc.), Virginia Commonwealth University
• Inventors: Carr, Marcus E., Stevens, Peter J., Simpson, David G., Bowlin, Gary L., Wnek, Gary E., Rajendran, Saravanamoorthy, Matthews, Jamil A.
• Primary Examiner(s): Sweet; Thomas J
• Application Number:
  US10/447,670
• Publication Number:
  US 20040037813A1
• Time in Patent Office: 2,358 Days
• Field of Search: 623/14.13, 623/23.72, 435/398
• US Class Current: 435/398
• CPC Class Codes: A61F 2/08 Muscles; Tendons; Ligaments sutures A61B17/04
• CPC Class Codes: A61K 2035/126 Immunoprotecting barriers, e.g. jackets, diffusion chambers
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61L 15/32 Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin A61L15/225 takes precedence
• CPC Class Codes: A61L 2430/30 for muscle reconstruction
• CPC Class Codes: A61L 27/18 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/26 Mixtures of macromolecular compounds
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 27/3804 characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
• CPC Class Codes: A61L 27/3839 characterised by the site of application in the body materials for artificial blood vessels A61L27/507; materials for use in artificial skin A61L27/60
• CPC Class Codes: A61L 27/3895 using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
• CPC Class Codes: A61L 27/507 for artificial blood vessels apparatus for applying cells on a blood vessel prosthesis A61F2/062
• CPC Class Codes: A61L 31/041 Mixtures of macromolecular compounds
• CPC Class Codes: A61P 41/00 Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution
• CPC Class Codes: B29C 41/006 using an electrostatic field for applying the material
• CPC Class Codes: B29C 67/0007 Manufacturing coloured articles not otherwise provided for, e.g. by colour change
• CPC Class Codes: C07K 14/78 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• CPC Class Codes: C08L 67/04 Polyesters derived from hydroxycarboxylic acids, e.g. lactones C08L67/06 takes precedence
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00
• CPC Class Codes: C08L 89/06 : derived from leather or skin , e.g. gelatin
• CPC Class Codes: C12N 2533/40 : Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
• CPC Class Codes: C12N 2533/54 : Collagen; Gelatin
• CPC Class Codes: C12N 5/0012 : Cell encapsulation
• CPC Class Codes: C12N 5/0068 : General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: D01D 5/0038 : the fibre formed by solvent evaporation, i.e. dry electro-spinning
• CPC Class Codes: D01F 1/10 : Other agents for modifying properties
• CPC Class Codes: D01F 4/00 : Monocomponent artificial filaments or the like of proteins; Manufacture thereof
• CPC Class Codes: Y10T 428/298 : Physical dimension
• CPC Class Codes: Y10T 442/2525 : Coating or impregnation functions biologically [e.g., insect repellent, antiseptic, insecticide, bactericide, etc.]
• CPC Class Codes: Y10T 442/60 : Nonwoven fabric [i.e., nonwoven strand or fiber material]
• CPC Class Codes: Y10T 442/614 : Strand or fiber material specified as having microdimensions [i.e., microfiber]

Abstract

The invention is directed to formation and use of electroprocessed collagen, including use as an extracellular matrix and, together with cells, its use in forming engineered tissue. The engineered tissue can include the synthetic manufacture of specific organs or tissues which may be implanted into a recipient. The electroprocessed collagen may also be combined with other molecules in order to deliver substances to the site of application or implantation of the electroprocessed collagen. The collagen or collagen/cell suspension is electrodeposited onto a substrate to form tissues and organs.

Claims

1. Synthetic electrospun collagen fibers electrospun from a solvent comprising a halogenated alcohol comprising a repeated longitudinal banding pattern, wherein the repeated banding pattern occurs at a spacing of about 65 nm to about 67 nm;

wherein the synthetic electrospun collagen fibers comprise a matrix and the matrix has an elastic modulus between about 0.5 and about 10 MPa when hydrated.

2. The electrospun collagen fibers of claim 1, wherein the electrospun collagen fibers have an average diameter of between about 30 nm and about 10 μ

m.

26. The synthetic collagen fibers of claim 1 or composition of any of claims 3, 11, or 19 wherein the halogenated alcohol is selected from the group consisting of a fluorinated alcohol, hexafluoroacetone and a chloroalcohol.

3. A composition comprising electroprocessed collagen comprising a repeated longitudinal banding pattern, wherein the repeated banding pattern occurs at a spacing of about 65 nm to about 67 nm;

and,

wherein the electroprocessed collagen comprises synthetic electrospun collagen fibers electrospun from a solvent comprising a halogenated alcohol.

4. The composition of claim 3, wherein the electroprocessed collagen is selected from the group consisting of collagen type I, II, III, IV, V, VI, VII, VIII, IX, X, XI, XII, XIII, XIV, XV, XVI, XVII, XVIII, XIX and a combination thereof.

5. The composition of claim 3, wherein the electroprocessed collagen is selected from the group consisting of collagen type I, II, III and a combination thereof.

6. The composition of claim 3, wherein the repeated longitudinal banding pattern occurs at a spacing of about 67 nm.

7. The composition of claim 3, wherein the electroprocessed collagen comprises fibers having a diameter of about 10 nm to about 100 μ

m.

8. The composition of claim 3, further comprising one or more substances.

9. The composition of claim 3, further comprising additional electroprocessed material selected from the group consisting of one or more natural materials, one or more synthetic materials, and a combination thereof.

10. The composition of claim 3, further comprising one or more polymers.

11. A composition comprising synthetic electroprocessed collagen in a matrix, the matrix comprising a pore size in a range of about 0.2 microns to about 2.3 microns;

wherein the electroprocessed collagen comprises synthetic electrospun collagen fibers electrospun from a solvent comprising a halogenated alcohol and comprising a repeated longitudinal banding pattern; and

,

wherein the repeated banding pattern occurs at a spacing of about 65 nm to about 67 nm.

12. The composition of claim 11, further comprising one or more substances.

13. The composition of claim 12, wherein the one or more substances is cells.

14. The composition of claim 12, wherein the one or more substances is selected from the group consisting of elastin, laminin, integrin, proteoglycan, and a combination thereof.

15. The composition of claim 11, the matrix further comprising additional electroprocessed material selected from the group consisting of one or more natural materials, one or more synthetic materials, and a combination thereof.

16. The composition of claim 11, further comprising one or more polymers.

17. The composition of claim 11, the matrix having a thickness of about 200 microns to about 250 microns.

18. The composition of claim 11, the matrix having a thickness of about 50 microns.

19. A composition comprising electroprocessed collagen comprising a blend of at least two types of collagen;

and,

wherein the electroprocessed collagen comprises synthetic electrospun collagen fibers electrospun from a solvent comprising a halogenated alcohol.

20. The composition of claim 19, wherein the at least two types of collagen are selected from the group consisting of collagen type I, II, III, IV, V, VI, VII, VIII, IX, X, XI, XII, XIII, XIV, XV, XVI, XVII, XVIII and XIX.

21. The composition of claim 19, wherein the at least two types of collagen are selected from the group consisting of natural collagen, procollagen, genetically modified collagen, chemically modified collagen, and a combination thereof.

22. The composition of claim 19, further comprising one or more polymers.

23. The composition of claim 19, comprising a pore size of about 10 microns or less.

24. The composition of claim 19, further comprising one or more substances.

25. The composition of claim 19, further comprising additional electroprocessed material comprising one or more natural materials, one or more synthetic materials, or a combination thereof.