Modified cottonseed oil

• US 7,619,105 B2
• Filed: 08/12/2005
• Issued: 11/17/2009
• Est. Priority Date: 04/18/2000
• Status: Expired due to Term

• Current Assignee: Commonwealth Scientific & Industrial Research Organisation (Commonwealth Of Australia As Represented By Department Of Industry, Innovation, Climate Change, Science, Research And Tertiary Education)
• Original Assignee: Commonwealth Scientific & Industrial Research Organisation (Commonwealth Of Australia As Represented By Department Of Industry, Innovation, Climate Change, Science, Research And Tertiary Education)
• Inventors: Liu, Qing, Green, Allan, Singh, Surinder
• Primary Examiner(s): Carr; Deborah D
• Application Number:
  US11/202,731
• Publication Number:
  US 20060037104A1
• Time in Patent Office: 1,558 Days
• Field of Search: None
• US Class Current: 554/227
• CPC Class Codes: C12N 15/8247 involving modified lipid metabolism, e.g. seed oil composition
• CPC Class Codes: C12N 9/0083 Miscellaneous (1.14.99)

Abstract

The present invention provides novel gene constructs and methods for the production of transgenic cotton plants that produce oils having a range of desirable attributes, including improved oil quality, and modified fatty acid composition.

Claims

1. A cotton seed-derived oil having a palmitic acid content which is decreased by 25.4% relative to non-tranformed G. hirsutum Coker cotton seed-derived oil.

2. The cotton seed-derived oil of claim 1, wherein the palmitic acid content is decreased by 35% to 37%.

3. The cotton seed-derived oil of claim 1, wherein the palmitic acid content is decreased to approximately 50% of the level of the non-tranformed G. hirsutum Coker cotton seed-derived oil.

4. The cotton seed-derived oil of claim 1, having an increased stearic acid content relative to the non-transformed G. hirsutum Coker cotton seed-derivel oil.

5. The cotton seed-derived oil of claim 4, wherein the stearic acid content is between 3% and 35% of the total fatty acid content in the oil.

6. The cotton seed-derived oil of claim 4, wherein the stearic acid content is up to approximately 40% of the total lipid content in the oil.

7. The cotton seed-derived ail of claim 4, further having an increased oleic acid content relative to the non-tranformed G. hirsutum Coker cotton seed-derived oil.

8. The cotton seed-derived nil of claim 7, further having a decreased linoleic acid content relative to the non-tranformed G. hirsutum Coker cotton seed-derived oil.

9. The cotton seed-derived oil of claim 1, having an increased oleic acid content relative to the non-tranformed G. hirsutum Coker cotton seed-derived oil.

10. The cotton seed-derived oil of claim 9, wherein the oleic acid content is between 16% and 75% of the total fatty acid content in the oil.

11. The cotton seed-derived oil of claim 9, wherein the oleic acid content is up to approximately 80% of the total lipid content in the oil.

12. The cotton seed-derived oil of claim 9, further having a decreased linoleic acid content relative to the non-tranformed G. hirsutum Coker cotton seed-derived oil.

13. The cotton seed-derived oil of claim 1, having a decreased linoleic acid content relative to the non-transformed G. hirsutum Coker cotton seed-derived oil.

14. The cotton seed-derived oil of claim 13, wherein the linoleic acid content is decreased by up to about 25%.

15. The cotton seed-derived oil of claim 13, wherein the linoleic acid content is decreased by more than 50%.

16. The cotton seed-derived oil of claim 13, wherein the linoleic acid content is decreased by up to about 95%.

17. The cotton seed-derived oil of claim 13, further having an increased stearic acid content relative to the non-tranformed G. hirsutum Coker cotton seed-derived oil.

18. A process for extracting the cotton seed-derived oil of claim 1, comprising the step of crushing a cotton seed, so as to thereby obtain the oil.

19. The process of claim 18, further comprising growing a cotton plant and harvesting the cotton seed.

20. A method of preparing food comprising the step of using the cotton seed-derived oil of claim 1 as a food ingredient or as a cooking oil.

21. A modified endogenous cottonseed oil having a palmitic acid content which is decreased by 25.4% relative to non-tranformed G. hirsutum coker cottonseed oil.

22. The modified endogenous cottonseed oil of claim 21, wherein the palmitic acid content is decreased by up to about 35% to 37%.

23. The modified endogenous cottonseed oil of claim 21, wherein the palmitic acid content is decreased to approximately 50% of the level of the non-tranformed G. hirsutum Coker cottonseed oil.

24. The modified endogenous cottonseed oil of claim 21, having an increased stearic acid content relative to the non-tranformed G. hirsutum Coker cottonseed oil.

25. The modified endogenous cottonseed oil of claim 24, wherein the stearic acid content is between 3% and 35% of the total fatty acid content in the oil.

26. The modified endogenous cottonseed oil of claim 24, wherein the stearic acid content is up to approximately 40% of the total lipid content in the oil.

27. The modified endogenous cottonseed oil of claim 24, further having an increased oleic acid content relative to the non-tranformed G. hirsutum Coker cottoned oil.

28. The modified endogenous cottonseed oil of claim 27, further having a decreased linoleic acid content relative to the non-tranformed G. hirsutum Coker cottonseed oil.

29. The modified endogenous cottonseed oil of claim 21, having an increased oleic acid content relative to the non-tranformed G. hirsutum Coker cottonseed oil.

30. The modified endogenous cottonseed oil of claim 29, wherein the oleic acid content is between 16% and 75% of the total fatty acid content in the oil.

31. The modified endogenous cottonseed oil of claim 29, wherein the oleic acid content is up to approximately 80% of the total lipid content in the oil.

32. The modified endogenous cottonseed oil of claim 29, further having a decreased linoleic acid content relative to the non-tranformed G. hirsutum Coker cottonseed oil.

33. The modified endogenous cottonseed oil of claim 21, having a decreased linoleic acid content relative to the non-tranformed G. hirsutum Coker cottonseed oil.

34. The modified endogenous cottonseed oil of claim 33, wherein the linoleic acid content is decreased by up to about 25%.

35. The modified endogenous cottonseed oil of claim 33, wherein the linoleic acid content is decreased by more than 50%.

36. The modified endogenous cottonseed oil of claim 33, wherein the linoleic acid content is decreased by up to about 95%.

37. The modified endogenous cottonseed oil of claim 33, further having an increased stearic acid content relative to the non-transformed G. hirsutum Coker cottonseed oil.

38. The modified endogenous cottonseed oil of claim 21, wherein the oil is present in a cotton seed.

39. A process for extracting the modified endogenous cottonseed oil of claim 21, comprising the step of crushing a cotton seed, so as to thereby obtain the oil.

40. A method of preparing food comprising the step of mixing the modified endogenous cottonseed oil of claim 21 with a food ingredient, thereby preparing the food.