Optical measuring device for test strips

• US 7,623,240 B2
• Filed: 11/18/2002
• Issued: 11/24/2009
• Est. Priority Date: 11/20/2001
• Status: Active Grant

• Current Assignee: Beckman Coulter GmbH (Danaher Corporation)
• Original Assignee: Iris Deutschland GmbH
• Inventors: Rudolf, Heiko
• Primary Examiner(s): PUNNOOSE, ROY M
• Application Number:
  US10/496,356
• Publication Number:
  US 20060176483A1
• Time in Patent Office: 2,563 Days
• Field of Search: 356/39, 356/402, 356/429, 356/424
• US Class Current: 356/402
• CPC Class Codes: G01N 21/255 Details, e.g. use of specially adapted sources, lighting or optical systems
• CPC Class Codes: G01N 21/276 with alternation of sample and standard in optical path
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04

Abstract

An optical measuring device (10) for detecting the coloring of test fields of a test strip (12), which is to be wetted with a liquid for detecting substances in said liquid, whereupon the reflectivity of the test fields changes depending on the concentrations of the substances to be detected, comprising: a measuring plane (14) wherein said test strip (12) is to be placed; an illumination device (16) for illuminating the measuring plane (14); a planar image sensor (36); an optical system for imaging said measuring plane (14) onto the image sensor (36); and an electronic evaluation unit (52) for evaluation of the signals detected by said image sensor (36), said illumination device (16) comprises light sources (18, 20, 22) or other means of different colors which serve to alternatively illuminate said measuring plane (14) in different colors, wherein said electronic evaluation unit (52) detects the coloring of the test fields from the images obtained under different color illuminations.

Claims

1. An optical measuring device for detecting the coloring of test fields of a test strip to be wetted with a liquid for detecting substances in said liquid, whereupon the reflectivity of the test fields changes depending on the concentrations of the substances to be detected, comprising:

a measuring plane upon which said test strip is to be placed;

an illumination device for illuminating the measuring plane and the test strip;

a planar image sensor;

an optical system for providing an image of the test strip onto said planar image sensor;

an electronic evaluation unit for evaluating at least one signal generated by said planar image sensor, wherein said illumination device comprises at least two light sources of different colors to alternatively illuminate said test strip in said different colors such that said electronic evaluation unit detects the coloring of said test fields under different color illuminations; and

an adjustable reference surface that is movable between a first position in which it takes on the position of a test strip during measurement and a second position in which it cannot be imaged by said optical system onto said image sensor.

2. An optical measuring device according to claim 1, wherein said image sensor comprises light sensitive CMOS elements arranged in a planar matrix.

3. An optical measuring device according to claim 1 or 2, wherein said light sources of said illumination device comprise light emitting diodes (LEDs).

4. An optical measuring device according to claim 3, wherein said LEDs comprise blue, green and orange LEDs having approximate wavelengths of 450 nm, 530 nm and 620 nm, respectively.

5. An optical measuring device according to claim 1, wherein said light sources are arranged in a series on a transmitter board.

6. An optical measuring device according to claim 5, wherein the arrangement density of said light sources on the transmitter board increases from near the center of the transmitter board in an outward direction towards the ends of the transmitter board.

7. An optical measuring device according to claims 5 or 6, further comprising a screen on each side of the series of light sources, wherein said screens are disposed in parallel to the longitudinal direction of a test strip so as to focus light reflected from the light sources onto the test strip.

8. An optical measuring device according to claim 7, wherein said screens substantially extend over the length of the series of light sources and comprise a plurality of surface segments extending over the entire length od said screens, wherein said surface segments'"'"' inclination angle relative to said transmitter board increases in correspondence with an increasing distance from the transmitter board.

9. An optical measuring device according to claim 7, wherein said screens comprise parts having been milled or manufactured by injection molding, said parts having a reflecting layer or being laminated with a reflecting film.

10. An optical measuring device according to claim 1, further comprising a reference surface, different from the adjustable reference surface, that is disposed such that it is illuminated by said illumination device simultaneously with said test strip and imaged by said optical system onto said image sensor.

11. An optical measuring device according to claim 1, wherein said adjustable reference surface comprises a surface of a strip-shaped plate whose first end is equipped with a first arm substantially perpendicular with respect to said adjustable reference surface, said first arm being pivotable about an axis parallel with respect to said adjustable reference surface.

12. An optical measuring device according to claim 11, further comprising a second arm disposed at a second end of the strip-shaped plate, said second arm being substantially parallel with respect to the first arm and being pivotable about the same axis as the first arm.

13. An optical measuring device according to claims 11 or 12, further comprising a bar pivotally supported by the first arm at the bar'"'"'s first end for moving the adjustable reference surface from its first to its second position.

14. An optical measuring device according to claim 13, wherein said bar is pivotally secured at its second end to a first lever, said first lever being pivotable about a first lever axis and being biased into a first lever position by means of a biasing element such that said adjustable reference surface takes its second position and wherein said first lever is adjustable to a second lever position against the biasing force of said biasing element such that said adjustable reference surface takes its first position.

15. An optical measuring device according to claim 14, wherein said first lever is actuated by means of an eccentric drive.

16. An optical measuring device according to claim 15, further comprising means for holding and aligning a test strip in its measuring position.

17. An optical measuring device according to claim 16, wherein said means for holding and aligning comprise two substantially parallel pins movable along their longitudinal axis between a first position in which they protrude into said measuring plane and a second position in which they are entirely outside said measuring plane.

18. An optical measuring device according to claim 17, wherein said pins are biased into their second position and being adjustable to their first position against the biasing force by means of a lever element.

19. An optical measuring device according to claim 18, wherein said lever element is adjusted by the same eccentric drive as the first lever.

20. An optical measuring device according to claim 19, wherein said eccentric drive comprises a rotating disc driven by a motor, and a second pin arranged perpendicularly on a surface of said rotating disc such that upon rotation of the disc in a first direction of rotation, said second pin moves said first lever into its second lever position and upon rotation of the disc in a second direction of rotation, said pin moves said lever element such that said lever element moves said substantially parallel pins into their first position.