Transformed bacteria producing CS6 antigens as vaccines

• US 7,625,571 B2
• Filed: 03/06/2007
• Issued: 12/01/2009
• Est. Priority Date: 05/13/1994
• Status: Expired due to Fees

• Current Assignee: United States Army (U.S. Department Of Defense)
• Original Assignee: The United States Of America As Represented By The Secretary Of The Army
• Inventors: Wolf, Marcia Kay, Boedeker, Edgar Charles, Cassels, Frederick J.
• Primary Examiner(s): Graser; Jennifer E
• Application Number:
  US11/682,648
• Publication Number:
  US 20080038319A1
• Time in Patent Office: 1,001 Days
• Field of Search: None
• US Class Current: 424/241.1
• CPC Class Codes: A61K 2039/523 expressing foreign proteins
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: C07K 14/245 Escherichia (G)

Abstract

Disclosed herein are antigens that stimulate protective antibodies against enterotoxigenic Escherichia coli. Also disclosed herein are proteins encoded by cssA and cssB genes as well as constructs containing the genes and methods of using thereof.

Claims

1. A purified protein having the amino acid sequence set forth in SEQ ID NO:

7.

2. A composition comprising the protein of claim 1.

3. A method of inducing an antigenic response in a subject which comprises administering to the subject the protein of claim 1.

4. The method of claim 3, wherein the protein is administered in the form of a host cell that expresses the protein.

5. The method of claim 3, wherein the host cell is a bacterial strain.

6. The method of claim 5, wherein the bacterial strain is attenuated or the bacterial strain is killed after the protein is expressed.

7. The method of claim 3, wherein the protein is administered to the subject in the form of a drink.

8. The method of claim 7, wherein the drink is a carbonated beverage.

9. The method of claim 3, wherein the protein is orally administered.

10. An antigenic composition comprising at least one protein of claim 1.

11. The antigenic composition of claim 10, and further comprising at least one CssB protein, at least one CssA protein, or both.