Methods and compositions for identifying bacteria associated with bacteria vaginosis

• US 7,625,704 B2
• Filed: 12/01/2006
• Issued: 12/01/2009
• Est. Priority Date: 08/31/2005
• Status: Active Grant

• Current Assignee: Fred Hutchinson Cancer Research Center (Fred Hutchinson Cancer Research Center|Argus Genetics|Mars)
• Original Assignee: Fred Hutchinson Cancer Research Center (Fred Hutchinson Cancer Research Center|Argus Genetics|Mars)
• Inventors: Fiedler, Tina, Fredricks, David N.
• Primary Examiner(s): Horlick; Kenneth R.
• Assistant Examiner(s): Tung; Joyce
• Application Number:
  US11/607,639
• Publication Number:
  US 20070178495A1
• Time in Patent Office: 1,096 Days
• Field of Search: 435/6, 435/91.2, 536/22.1, 536/24.3
• US Class Current: 435/6.15
• CPC Class Codes: C12Q 1/689 for bacteria

Abstract

The present invention provides methods and compositions for identifying bacteria associated with bacterial vaginosis and diagnosing bacterial vaginosis in a subject.

Claims

1. A method of detecting Megasphaera in a sample, comprising:

a) contacting the sample with a primer pair specific for nucleic acid comprising the nucleotide sequence of SEQ ID NO;

64 under conditions whereby nucleic acid amplification can occur; and

b) detecting amplification of the nucleic acid of (a), thereby detecting Megasphaera in the sample, wherein the primer pair comprises

     1) a forward primer comprising the nucleotide sequence of SEQ ID NO;

19, and

     2) a reverse primer comprising the nucleotide sequence of SEQ ID NO;

20.

2. A method of detecting Megasphaera in a sample, comprising:

a) contacting the sample with a primer pair specific for nucleic acid comprising the nucleotide sequence of SEQ ID NO;

64 under conditions whereby nucleic acid amplification can occur; and

b) detecting amplification of the nucleic acid of (a), thereby detecting Megasphaera in the sample, wherein the primer pair comprises

     1) a forward primer comprising the nucleotide sequence of (SEQ ID NO;

106, ) and

     2) a reverse primer comprising the nucleotide sequence of SEQ ID NO;

107.

3. A method of determining the amount of Megasphaera in a sample, comprising:

a) contacting the sample with a primer pair specific for nucleic acid comprising the nucleotide sequence of SEQ ID NO;

64 under conditions whereby nucleic acid amplification can occur and the amount of amplified nucleic acid can be determined; and

b) detecting amplification of the nucleic acid of (a) and determining the amount of Megasphaera nucleic acid and the amount of Megasphaera bacteria in the sample, wherein the primer pair comprises

     1) a forward primer comprising the nucleotide sequence of SEQ ID NO;

106, and

     2) a reverse primer comprising, the nucleotide sequence of SEQ ID NO;

107 and the amplification of nucleic acid of (a) is detected by contacting the nucleic acid of (a) with a detectably labeled probe comprising the nucleotide sequence of SEQ ID NO;

108.