Waveguide grating structure and optical measurement arrangement

• US 7,627,201 B2
• Filed: 07/06/2004
• Issued: 12/01/2009
• Est. Priority Date: 08/09/2000
• Status: Active Grant

• Current Assignee: Artificial Sensing Instruments ASI AG
• Original Assignee: Artificial Sensing Instruments ASI AG
• Inventors: Tiefenthaler, Kurt
• Primary Examiner(s): Font; Frank G
• Assistant Examiner(s): CHIEM, DINH D
• Application Number:
  US10/885,449
• Publication Number:
  US 20040247229A1
• Time in Patent Office: 1,974 Days
• Field of Search: 385/12
• US Class Current: 385/12
• CPC Class Codes: G01N 2021/6463 Optics
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: G01N 2201/08 Optical fibres; light guides
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G02B 2006/12107 Grating
• CPC Class Codes: G02B 6/124 Geodesic lenses or integrated gratings
• CPC Class Codes: G02B 6/32 having lens focusing means positioned between opposed fibre ends with lens being an integral part of the single fibre end G02B6/262
• CPC Class Codes: Y10S 385/901 Illuminating or display apparatus

Abstract

The present invention describes (bio)chemo-functional waveguide grating structures consisting of at least one (bio)chemo-functional waveguide grating structure unit or at least one (bio)chemo-functional sensor location with beam guidance permitting light beam separation, as well as detection methods for parallel analysis which are marking-free or based on marking.

Claims

1. An optical sensor for sensing at least one chemical or biochemical substance or for studying the behavior of cells, comprising

a broad band light source for producing an incident light field,

a sensor chip which includes a waveguide grating structure on a substrate, the waveguide grating structure comprising an array of waveguide grating structure units or an array of sensor locations;

the waveguide grating structure being at least partially covered by at least one chemosensitive or biochemosensitive substance or by cells;

the waveguide grating structure comprising at least one waveguide and at least one grating, and the waveguide grating structure being capable of emitting at least one light field when the incident light field at least partially illuminates the waveguide grating structure to yield an emitted light field that propagates away from said waveguide;

a grating or prism spaced apart from the waveguide grating structure and arranged in the emitted light field propagating away from said waveguide, so as to influence the emitted light field in a wavelength dependent manner, thereby yielding an influenced light field; and

a detector device comprising an array of detectors arranged to detect at least portions of the influenced light field.

2. The optical sensor of claim 1, the detector device being capable of detecting light distribution changes.

3. The optical sensor of claim 1, comprising a linker layer or modified surface arranged between the waveguide grating structure and the chemosensitive or biochemosensitive substance or cells.

4. The optical sensor of claim 1, wherein the waveguide grating structure is illuminated at a fixed angle.

5. The optical sensor of claim 1, wherein the sensor chip comprises a plastic substrate, and wherein an inorganic layer is arranged between the waveguide and the plastic substrate.

6. The optical sensor of claim 1, wherein the array of detectors is a one dimensional or two dimensional pixel array detector.

7. The optical sensor of claim 1, wherein the array of detectors is a one dimensional or two dimensional array of position sensitive detectors.

8. The optical sensor of claim 1, wherein the waveguide grating structure units or sensor locations comprise at least one chemosensitive or biochemosensitive sensing pad or sensing pad at least partially covered by cells.

9. The optical sensor of claim 8, wherein the chemosensitive or biochemosensitive substance is provided as a chemosensitive or biochemosensitive layer, said layer having a thickness of less than a wavelength.

10. The optical sensor of claim 8, wherein the waveguide grating structure comprises at least one chemosensitive or biochemosensitive signal sensing pad or signal sensing pad at least partially covered by cells and at least one reference sensing pad.

11. The optical sensor of claim 10, wherein the reference sensing pad is capable of suppressing or nearly suppressing non-specific binding.

12. The optical sensor of claim 1, wherein at least one liquid holder or sample accommodating device is affixed onto the waveguide grating structure or wherein the waveguide grating structure is inserted into at least one liquid holder or sample accommodating device.

13. The optical sensor of claim 12, wherein the at least one liquid holder or sample accommodating device comprises at least one well or at least one flow-through cuvette or at least one capillary cuvette or at least one lab-on-chip.

14. The optical sensor of claim 12 comprising a one-dimensional or two-dimensional array or circular ring arrangement of liquid holders or sample accommodating devices.

15. The optical sensor of claim 14, wherein the liquid holders or sample accommodating devices are formed by a well plate.

16. The optical sensor of claim 12, wherein a matrix-like arrangement of a plurality of chemosensitive or biochemosensitive substances or cells is located below or in said liquid holder or sample accommodating device or below or in at least one of said liquid holders or sample accommodating devices.

17. The optical sensor of claim 12, wherein the liquid holder or sample accommodating device is at least partially made of scatter radiation absorbing material.

18. The optical sensor of claim 12, wherein the waveguide grating structure units or sensor locations comprise at least one signal sensing pad and at least one reference sensing pad and wherein the signal sensing pad and the reference sensing pad belong to the same liquid holder or sample accommodating device or belong to different liquid holders or sample accommodating devices.

19. The optical sensor of claim 1, wherein the waveguide grating structure comprises a stronger modulated waveguide grating.

20. The optical sensor of claim 1, wherein the waveguide grating structure comprises at least two waveguide gratings.

21. The optical sensor of claim 1, wherein the waveguide grating structure comprises at least two layers, wherein a waveguide grating is located at least at the interface between two of said layers.

22. The optical sensor of claim 1, wherein a lens system or an array of lens systems or collimation optics is located between the light source and the waveguide grating structure.

23. The optical sensor of claim 1, wherein a lens system or an array of lens systems is located between the waveguide grating structure and the detector device.

24. The optical sensor of claim 1, wherein the waveguide grating structure comprises at least one unidiffractive or bidiffractive or multidiffractive grating.

25. The optical sensor of claim 24, wherein the waveguide grating is coated at least partially by the chemosensitive orbiochemosensitive substance or the cells.

26. The optical sensor of claim 24, wherein the waveguide grating structure includes waveguide structure regions between two waveguide gratings and is coated by the chemosensitive or biochemosensitive substance or by the cells at least partially in at least one of said waveguide structure regions between two waveguide gratings.

27. The optical sensor of claim 1, wherein the waveguide grating structure is capable of guiding the transverse electric mode with mode number 0 or the transverse magnetic mode with mode number 0 or the transverse electric mode with mode number 0 and the transverse magnetic mode with mode number 0.

28. The optical sensor of claim 1, wherein the chemosensitive or biochemosensitive substance or at least one of said chemosensitive or biochemosensitive substances, respectively, is capable of binding specifically or non-specifically or specifically and non-specifically at the surface or in the volume or at the surface and in the volume.

29. The optical sensor of claim 1, wherein the waveguide grating structure is a chemosensitive and biochemosensitive waveguide grating structure, and wherein the chemosensitive or biochemosensitive substance is a chemosensitive and biochemosensitive substance.

30. The optical sensor of claim 1, wherein the waveguide grating structure is at least partially covered by living cells.

31. The optical sensor of claim 1, wherein the chemosensitive or biochemosensitive substance comprises a molecular imprinted polymer.

32. The optical sensor of claim 1, wherein at least one waveguide grating structure unit or sensor location comprises at least one chemosensitive or biochemosensitive signal sensing pad or signal sensing pad at least partially covered by cells and at least one reference sensing pad.