Sequential analysis of biological samples
First Claim
1. A method of detecting multiple targets in a biological sample, comprising:
- (a) contacting the sample with a first probe;
(b) physically binding the first probe to a first target;
(c) observing a first signal from the first probe;
(d) applying a chemical agent after step (c) to modify the observed first signal;
(e) contacting the sample with a second probe;
(f) physically binding the second probe to a second target; and
(g) observing a second signal from the second probe.
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Accused Products
Abstract
Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.
38 Citations
31 Claims
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1. A method of detecting multiple targets in a biological sample, comprising:
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(a) contacting the sample with a first probe; (b) physically binding the first probe to a first target; (c) observing a first signal from the first probe; (d) applying a chemical agent after step (c) to modify the observed first signal; (e) contacting the sample with a second probe; (f) physically binding the second probe to a second target; and (g) observing a second signal from the second probe. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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28. A method of detecting multiple targets in a biological sample, comprising:
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(a) contacting the sample with a first probe; (b) physically binding the first probe to a first target; (c) observing a first signal from the first probe; (d) coffelating the observed first signal to the detection of the first target; (e) applying a chemical agent after step (d) to modify the observed first signal in step (c) ; and (f) repeating the steps (a) to (e) with a second probe, a third probe, or an nth probe to detect a second target, a third target, or an nth target. - View Dependent Claims (29, 31)
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30. A method of detecting multiple targets in a biological sample, comprising:
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(a) contacting the biological sample with a first probe, wherein the first probe is capable of binding a first target; (b) physically binding the first probe to the first target to form a first target-bound probe; (c) observing a first signal from the first target-bound probe; (d) applying a chemical agent after step (c) to decrease the observed first signal; (e) correlating the observed first signal to the detection of the first target; and (f) repeating the steps (a) to (e) with a subsequent probe to detect a subsequent target.
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Specification