Disposable bioreactor systems and methods
First Claim
1. A bioreactor system comprising:
- a disposable container for housing biomaterials for processing, the disposable container comprising a single chamber including at least one input port;
a fitting comprising a porous surface associated with the input port and configured for allowing the passage of an inlet gas stream and controlling gas bubble size and distribution prior to addition of the inlet gas stream to the interior of the single chamber, wherein the pore size of the porous surface is chosen from macro, micron, submicron, nano, and combinations thereof;
a disposable mixing system comprising an impeller positioned above the porous surface and within the single chamber at a lower portion of the single chamber, the impeller configured to be driven by a motor magnetically coupled to the impeller and external to the lower portion of the single chamber such that biomaterials contained within the single chamber are mixed and gas bubble circulation is increased;
at least one exhaust port;
at least one harvest port;
a structure for supporting the disposable container;
one or more sensors for sensing one or more parameters of the biomaterials in the container; and
a heater for heating the contents of the disposable container, the heater having a thermostat.
6 Assignments
0 Petitions
Accused Products
Abstract
Accordingly, in one embodiment of the invention, a bioreactor system is presented and includes a disposable container for housing biomaterials for processing, the disposable container including at least one input port, at least one exhaust port, at least one harvest port, and the integrity of the sterile environment is protected with sterile filters attached to all external open ports a structure for supporting the disposable container, one or more sensors for sensing one or more parameters of the biomaterials in the container, a heater for heating the contents of the container, the heater having a thermostat and mixing system arranged with the system such that biomaterials contained in the disposable container are mixed.
189 Citations
27 Claims
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1. A bioreactor system comprising:
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a disposable container for housing biomaterials for processing, the disposable container comprising a single chamber including at least one input port; a fitting comprising a porous surface associated with the input port and configured for allowing the passage of an inlet gas stream and controlling gas bubble size and distribution prior to addition of the inlet gas stream to the interior of the single chamber, wherein the pore size of the porous surface is chosen from macro, micron, submicron, nano, and combinations thereof; a disposable mixing system comprising an impeller positioned above the porous surface and within the single chamber at a lower portion of the single chamber, the impeller configured to be driven by a motor magnetically coupled to the impeller and external to the lower portion of the single chamber such that biomaterials contained within the single chamber are mixed and gas bubble circulation is increased; at least one exhaust port; at least one harvest port; a structure for supporting the disposable container; one or more sensors for sensing one or more parameters of the biomaterials in the container; and a heater for heating the contents of the disposable container, the heater having a thermostat. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 21, 22, 23, 24)
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14. A bioreactor system comprising:
a support structure; a disposable container comprising a single chamber positioned within the support structure, the disposable container including; an impeller plate affixed to a lower portion of the single chamber, the impeller plate including a post having an end projecting into the single chamber; a fitting attached to the impeller plate, the fitting comprising a porous surface arranged to allow one or two-way fluid communication between an interior of the single chamber and an exterior of the single chamber, the porous surface having a pore size chosen from macro, micron, submicron, nano, and combinations thereof; a disposable impeller hub mounted on the post, the disposable impeller hub having at least one disposable impeller blade and having at least one magnet, the at least one impeller blade positioned above the porous surface and arranged on the post; a motor having a shaft, the motor being provided adjacent to or within the support structure; and a motor hub mounted on the motor shaft, the motor hub including at least one magnet, wherein upon mounting of the disposable container comprising a single chamber within the support structure, the motor hub aligns with the impeller plate such that the magnet of the motor hub may align with the magnet of the disposable impeller hub to drive the disposable impeller hub when the motor shaft rotates. - View Dependent Claims (15, 16, 17, 18, 19, 20, 25, 26, 27)
Specification