Surfaces coated with target-induced fluorescent compounds for detection of target elements
First Claim
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1. A method for direct detection of a trace element, the method comprising:
- (a) linking a surface of a nanoparticle with a compound to form a nanoparticle-linked compound, wherein the compound is selected from a group consisting of;
2,3-diaminonapthalene (DAN);
3,3,′
-diaminobenzidine (DAB);
4-[bis(carboxymethyl)-aminomethyl]-3-hydroxy-2-naphthoic acid (DHNA);
acetic acid, 3,3′
-[vinylenebis[(6-hydroxy-m-phenylene)methylenenitrilo]]tetra-, E-(8CI) (BDDS);
8-amino-3,7,-dimethyl-10-phenyl-10H-phenazion-2-ylidene ammonium ion;
3-[(4-amino-2-methyl-5-pyrimidinyl)methyl]-5-(2-hydroxyethyl)-4-methylthiazolium (thiamine);
carboxymethyl-[4-[10-(4-dicarboxymethylaminobenzyl)anthracen-9-ylmethyl]phenyl]amino) acetic acid dipotassium salt (CDAPAP); and
NMBDA, wherein NMBDA is a compound of the formula;
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Abstract
Surfaces coated with a target-induced fluorescent compound are used to detect target elements, especially trace elements. The target-induced fluorescent compound does not fluoresce when excited at a specific wavelength until it has bound a target element. The use of these coated surfaces provides several benefits including reduced background for greater sensitivity and eliminating the need to separate target-induced fluorescent compound that has not bound target element from target-induced fluorescent compound that has bound target element. Coated nanoparticles are especially useful for detection of target elements.
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Citations
11 Claims
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1. A method for direct detection of a trace element, the method comprising:
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(a) linking a surface of a nanoparticle with a compound to form a nanoparticle-linked compound, wherein the compound is selected from a group consisting of; 2,3-diaminonapthalene (DAN); 3,3,′
-diaminobenzidine (DAB);4-[bis(carboxymethyl)-aminomethyl]-3-hydroxy-2-naphthoic acid (DHNA); acetic acid, 3,3′
-[vinylenebis[(6-hydroxy-m-phenylene)methylenenitrilo]]tetra-, E-(8CI) (BDDS);8-amino-3,7,-dimethyl-10-phenyl-10H-phenazion-2-ylidene ammonium ion; 3-[(4-amino-2-methyl-5-pyrimidinyl)methyl]-5-(2-hydroxyethyl)-4-methylthiazolium (thiamine); carboxymethyl-[4-[10-(4-dicarboxymethylaminobenzyl)anthracen-9-ylmethyl]phenyl]amino) acetic acid dipotassium salt (CDAPAP); and NMBDA, wherein NMBDA is a compound of the formula; - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method for direct detection of a trace element in the presence of an interfering element to produce a desired fluorescent signal, the method comprising:
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(a) forming a nanoparticle-linked compound, comprising; (1) forming a microemulsion containing a first compound; (2) forming a porous nanoparticle from the microemulsion, the first compound being trapped inside the porous nanoparticle, wherein the first compound is selected from a group consisting of; 2,3-diaminonapthalene (DAN) capable of binding selenium; 3,3,′
-diaminobenzidine (DAB) capable of binding selenium;4-[bis(carboxymethyl)-aminomethyl]-3-hydroxy-2-naphthoic acid (DHNA) capable of binding beryllium; acetic acid, 3,3′
-[vinylenebis[(6-hydroxy-m-phenylene)methylenenitrilo]]tetra-, E-(8CI) (BDDS) capable of binding cadmium;8-amino-3,7,-dimethyl-10-phenyl-10H-phenazion-2-ylidene ammonium ion capable of binding chromium; 3-[(4-amino-2-methyl-5-pyrimidinyl)methyl]-5-(2-hydroxyethyl)-4-methylthiazolium (thiamine) capable of binding mercury; carboxymethyl-[4-[10-(4-dicarboxymethylaminobenzyl)anthracen-9-ylmethyl]phenyl]amino) acetic acid dipotassium salt (CDAPAP) capable of binding zinc; and NMBDA capable of binding calcium, wherein NMBDA is a compound of the formula; - View Dependent Claims (9, 10, 11)
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Specification