Composite membrane containing a cross-linked enzyme matrix for a biosensor
First Claim
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1. A composite membrane for an enzyme sensor comprising:
- an inner membrane layer comprising a restorable polymerizable membrane, the inner membrane layer adapted to function as an interference rejection membrane;
an outer membrane layer comprising polyurethane, and adapted to be disposed adjacent a flow channel during use; and
an enzyme layer disposed between and in contact with the inner and the outer membrane layers, said enzyme layer comprising a matrix comprising;
at least one enzyme selected from the group consisting of glucose oxidase and lactate oxidase;
a cross-linking agent; and
an enzyme stabilizer selected from the group of polyionic compounds consisting of polyethyleneimine, polypropyleneimine, poly(N-vinylimidazole), polyallylamine, polyvinylpyridine, polyvinylpyrrolidone, polylysine, and protamine;
wherein the outer membrane is adapted to control diffusion of an analyte into the enzyme layer.
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Abstract
An electrochemical sensor system and membrane and method thereof for increased accuracy and effective life of electrochemical and enzyme sensors.
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Citations
16 Claims
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1. A composite membrane for an enzyme sensor comprising:
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an inner membrane layer comprising a restorable polymerizable membrane, the inner membrane layer adapted to function as an interference rejection membrane; an outer membrane layer comprising polyurethane, and adapted to be disposed adjacent a flow channel during use; and an enzyme layer disposed between and in contact with the inner and the outer membrane layers, said enzyme layer comprising a matrix comprising; at least one enzyme selected from the group consisting of glucose oxidase and lactate oxidase; a cross-linking agent; and an enzyme stabilizer selected from the group of polyionic compounds consisting of polyethyleneimine, polypropyleneimine, poly(N-vinylimidazole), polyallylamine, polyvinylpyridine, polyvinylpyrrolidone, polylysine, and protamine; wherein the outer membrane is adapted to control diffusion of an analyte into the enzyme layer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification