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Compositions and methods for propagation of neural progenitor cells

  • US 7,632,681 B2
  • Filed: 12/02/2004
  • Issued: 12/15/2009
  • Est. Priority Date: 12/02/2003
  • Status: Active Grant
First Claim
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1. A cell culture comprising:

  • (a) a culture medium, wherein the calcium concentration of the medium is about 0.03 mM to 0.15 mM;

    (b) about 20-100 ng/ml epidermal growth factor (EGF);

    (c) about 10-50 ng/ml basic fibroblast growth factor (bFGF);

    (d) about 1-150 ng/ml transforming growth factor-alpha (TGFα

    );

    (e) mammalian neural progenitor cells (NPC) immunopositive for Oct4 and nestin, and wherein the cells continue to proliferate for at least 1 year in vitro.

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