Method of predicting the onset of sepsis in SIRS-positive individuals using mass spectrometry
First Claim
1. A method of predicting the onset of sepsis in a systemic inflammatory response syndrome (“
- SIRS”
)-positive individual using mass spectrometry, comprising;
(a) obtaining a first biological sample taken from the SIRS-positive individual;
(b) pre-treating the first biological sample, wherein said pre-treating comprises releasing small molecules bound to proteins in the first biological sample, and(c) comparing (i) a first biomarker profile resolved from the pre-treated first biological sample using a mass spectrometry technique and (ii) a reference biomarker profile obtained from biological samples from a reference population;
wherein a single such comparison classifies the individual as belonging to or not belonging to the reference population;
wherein said individual'"'"'s first biomarker profile and said reference biomarker profile comprise a plurality of ions each having a mass-to-charge ratio of about 100 Daltons to about 1000 Daltons; and
wherein the comparison comprises applying a decision rule that predicts the onset of sepsis in the SIRS-positive individual where the reference population is selected from the group consisting of a SIRS-positive reference population confirmed as having sepsis after about 0-36 hours, a SIRS-positive reference population confirmed as having sepsis after about 36-60 hours, or a SIRS-positive reference population confirmed as having sepsis after about 60-84 hours.
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Abstract
Mass spectrometry techniques for determining the status of sepsis in an individual are provided. A biomarker profile resolved from a biological sample, taken from the individual, using a mass spectrometry technique is compared to a reference biomarker profile. A single such comparison classifies the individual as belonging to or not belonging to a reference population. The individual'"'"'s biomarker profile and the reference biomarker profile comprise a plurality of ions each having a mass-to-charge ratio of about 100 Daltons to about 1000 Daltons. The plurality of ions can be detected by electrospray ionization mass spectrometry in positive mode. The comparison uses a decision rule, such as a classification tree, that determines the status of sepsis in the individual without requiring knowledge of the identity of the biomarkers in the biomarker profile from the individual and without requiring knowledge of the identity of the biomarkers in the reference biomarker profile.
106 Citations
19 Claims
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1. A method of predicting the onset of sepsis in a systemic inflammatory response syndrome (“
- SIRS”
)-positive individual using mass spectrometry, comprising;(a) obtaining a first biological sample taken from the SIRS-positive individual; (b) pre-treating the first biological sample, wherein said pre-treating comprises releasing small molecules bound to proteins in the first biological sample, and (c) comparing (i) a first biomarker profile resolved from the pre-treated first biological sample using a mass spectrometry technique and (ii) a reference biomarker profile obtained from biological samples from a reference population;
wherein a single such comparison classifies the individual as belonging to or not belonging to the reference population;
wherein said individual'"'"'s first biomarker profile and said reference biomarker profile comprise a plurality of ions each having a mass-to-charge ratio of about 100 Daltons to about 1000 Daltons; and
wherein the comparison comprises applying a decision rule that predicts the onset of sepsis in the SIRS-positive individual where the reference population is selected from the group consisting of a SIRS-positive reference population confirmed as having sepsis after about 0-36 hours, a SIRS-positive reference population confirmed as having sepsis after about 36-60 hours, or a SIRS-positive reference population confirmed as having sepsis after about 60-84 hours. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
- SIRS”
Specification