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Method of predicting the onset of sepsis in SIRS-positive individuals using mass spectrometry

  • US 7,632,685 B2
  • Filed: 12/28/2006
  • Issued: 12/15/2009
  • Est. Priority Date: 11/12/2002
  • Status: Active Grant
First Claim
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1. A method of predicting the onset of sepsis in a systemic inflammatory response syndrome (“

  • SIRS”

    )-positive individual using mass spectrometry, comprising;

    (a) obtaining a first biological sample taken from the SIRS-positive individual;

    (b) pre-treating the first biological sample, wherein said pre-treating comprises releasing small molecules bound to proteins in the first biological sample, and(c) comparing (i) a first biomarker profile resolved from the pre-treated first biological sample using a mass spectrometry technique and (ii) a reference biomarker profile obtained from biological samples from a reference population;

    wherein a single such comparison classifies the individual as belonging to or not belonging to the reference population;

    wherein said individual'"'"'s first biomarker profile and said reference biomarker profile comprise a plurality of ions each having a mass-to-charge ratio of about 100 Daltons to about 1000 Daltons; and

    wherein the comparison comprises applying a decision rule that predicts the onset of sepsis in the SIRS-positive individual where the reference population is selected from the group consisting of a SIRS-positive reference population confirmed as having sepsis after about 0-36 hours, a SIRS-positive reference population confirmed as having sepsis after about 36-60 hours, or a SIRS-positive reference population confirmed as having sepsis after about 60-84 hours.

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