Hybrid phase lateral flow assay
First Claim
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1. An analytical device for a single-step determination of the presence of an analyte in a liquid sample, comprising:
- a non-porous solid surface comprising a sample reception zone and, in a reversible immobilization zone, a reversibly immobilized labeled reagent that binds said analyte to form a complex, anda porous carrier comprising, in a detection zone, an immobilized reagent that binds said complex in the presence of analyte to generate a detectable signal, wherein said porous carrier is in contact with said non-porous surface.
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Abstract
The invention relates to devices for performing single step assays for the determination of the presence or absence of an analyte in a liquid sample, and methods of determining the presence or absence of such analytes using such devices. Devices disclosed comprise a labeled analyte-binding reagent reversibly-immobilized on a non-porous solid material, which solid material is in physical contact with a dry porous carrier bearing an immobilized analyte-binding reagent. Also provided are quantitative assay devices.
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Citations
22 Claims
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1. An analytical device for a single-step determination of the presence of an analyte in a liquid sample, comprising:
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a non-porous solid surface comprising a sample reception zone and, in a reversible immobilization zone, a reversibly immobilized labeled reagent that binds said analyte to form a complex, and a porous carrier comprising, in a detection zone, an immobilized reagent that binds said complex in the presence of analyte to generate a detectable signal, wherein said porous carrier is in contact with said non-porous surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. An analytical device for the determination of an analyte in a liquid sample, comprising:
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a non-porous solid surface comprising a sample reception zone and, in a reversible immobilization zone, a reversibly immobilized reagent able to be mobilized with the passing liquid sample; and a porous carrier comprising, in a detection zone, an immobilized reagent that generates a detectable signal in the presence or absence of analyte, wherein said porous carrier is in contact with said non-porous surface.
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Specification