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Modified nucleic acid sequences and methods for increasing mRNA levels and protein expression in cell systems

  • US 7,632,980 B1
  • Filed: 10/20/1998
  • Issued: 12/15/2009
  • Est. Priority Date: 10/20/1997
  • Status: Expired due to Fees
First Claim
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1. A method for producing Plasmodium falciparum protein MSP-1 in milk of a non-human transgenic mammal, comprising:

  • 1) providing a non-human transgenic mammal whose genome comprises a modified nucleic acid sequence encoding said MSP-1 operably linked to a promoter which directs expression in a mammary gland and a signal sequence directing secretion of said MSP-1 into milk, wherein said modified nucleic acid sequence has been modified by replacing a number of AT-containing codons of a nucleic acid sequence encoding said MSP-1, as it naturally occurs in Plasmodium falciparum, with a codon or codons preferred by a mammalian cell for the purpose of expression and encoding the same amino acid as the replaced AT-containing codon or codons, wherein the number of codons replaced is sufficient to allow expression of said MSP-1 in said non-human transgenic mammal; and

    2) allowing said non-human transgenic mammal to express said MSP-1 in its milk.

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