Methods and devices for nucleic acid sequence determination
First Claim
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1. A method for conducting a chemical reaction on a surface, the method comprising the steps of:
- attaching a plurality of first nucleic acid molecules via covalent linkages to reactive chemical groups on a surface;
blocking the surface using a blocking solution comprising potassium phosphate;
conducting a chemical reaction between said first nucleic acid molecules and second reactant molecules comprising an optically detectable label wherein said conducting step comprises exposing said nucleic acid to a primer under conditions sufficient to extend said nucleic acid by at least one base;
rinsing unincorporated optically labeled nucleotides from the surface; and
observing individually optically resolvable labels incorporated in the one or more first nucleic acids on the surface.
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Abstract
Methods of the invention comprise methods and devices for nucleic acid sequence determination. Generally, the invention relates to preparing a substrate for sequencing a target nucleic acid.
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Citations
13 Claims
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1. A method for conducting a chemical reaction on a surface, the method comprising the steps of:
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attaching a plurality of first nucleic acid molecules via covalent linkages to reactive chemical groups on a surface; blocking the surface using a blocking solution comprising potassium phosphate; conducting a chemical reaction between said first nucleic acid molecules and second reactant molecules comprising an optically detectable label wherein said conducting step comprises exposing said nucleic acid to a primer under conditions sufficient to extend said nucleic acid by at least one base; rinsing unincorporated optically labeled nucleotides from the surface; and observing individually optically resolvable labels incorporated in the one or more first nucleic acids on the surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for conducting a chemical reaction on a surface, the method comprising the steps of:
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attaching a plurality of first nucleic acid molecules via covalent linkages to reactive chemical groups on an analytical surface; blocking the surface using a blocking solution comprising potassium phosphate; conducting a chemical reaction using second reactant molecules comprising an optically detectable label thereby incorporating said second reactant molecules; rinsing unincorporated optically labeled second reactant molecules from the surface; and observing individually optically resolvable labels on the surface.
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Specification