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Amplified signal in binding assays

  • US 7,635,571 B2
  • Filed: 12/07/2000
  • Issued: 12/22/2009
  • Est. Priority Date: 12/07/2000
  • Status: Active Grant
First Claim
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1. A method for determining the presence or concentration of an analyte in a medium, said method comprising:

  • providing a reaction mixture comprising in combination;

    a medium suspected of containing an analyte;

    a first specific binding pair member bound to a water-insoluble solid support;

    a second specific binding pair member bound to a sensitizer, said sensitizer capable in its excited state of generating a reactive oxygen species, wherein the proximity of the first specific binding pair member with the second specific binding pair member is modulated by the presence of the analyte; and

    digoxigenin-linked biotin linked to the solid support through a reactive oxygen cleavable linker;

    incubating the reaction mixture;

    exciting the sensitizer, said excitation of the sensitizer causing the formation of reactive oxygen, which cleaves the cleavable linker and releases digoxigenin-linked biotin from the solid support; and

    detecting the released digoxigenin-linked biotin, the amount thereof being related to the amount of analyte in said medium.

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