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Methods for conducting assays for enzyme activity on protein microarrays

  • US 7,635,572 B2
  • Filed: 06/09/2004
  • Issued: 12/22/2009
  • Est. Priority Date: 06/09/2003
  • Status: Active Grant
First Claim
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1. A method for assaying an enzymatic reaction, the method comprising:

  • (a) incubating at least one protein and at least one substance under conditions conducive to the occurrence of an enzymatic reaction between the protein and the substance, wherein (i) the protein and the substance are immobilized on the surface of a solid support, wherein the at least one protein and the at least one substance are immobilized on a solid support before the incubating step;

    (ii) the protein and the substance are in proximity sufficient for the occurrence of said enzymatic reaction; and

    (iii) the protein and the substance are not identical; and

    (b) determining whether said enzymatic reaction occurs, wherein the at least one substance is coated onto the solid support and the at least one protein is printed on the solid support.

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