Biosensor system
First Claim
1. Biosensor system for the electrochemical determination of an analyte concentration in a sample, the system comprisingan analyte reactant and at least two electrodes, wherein the analyte reactant is at least partially hydrated by the sample to form a reaction layer in contact with the electrodes for measurement of an analyte detection current flowing between the electrodes in response to an analysis voltage applied thereto, the analyte detection current corresponding to the analyte concentration in the sample by virtue of a reaction sequence comprising an analyte-specific reaction taking place in the reaction layer and an electrode reaction including transfer of electrons through a surface of one of the electrodes;
- andelectronic circuitry comprising a DC-voltage source for applying the analysis voltage to the electrodes and measurement and evaluation electronics for measuring a plurality of values along a curve of the analyte detection current versus time over a plurality of measurement times and for deriving the analyte concentration therefrom by means of an evaluation algorithm;
wherein the analysis voltage is applied to the electrodes during the analyte-specific reaction, the analyte-specific reaction and the electrode reaction taking place generally simultaneously and resulting in an analyte detection current versus time curve which comprises a rising section in which the current increases versus time; and
wherein the analyte detection current is measured at at least two points of time in the rising section and the values of the analyte detection current resulting from said at least two measurements are used in the evaluation algorithm for interferent error compensation.
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Accused Products
Abstract
Biosensor system and method for the electrochemical determination of an concentration of an analyte in a sample. An analyte reactant is at least partially hydrated by the sample to form a reaction layer in contact with electrodes for measurement of an analyte detection current. The analyte detection current is characteristic of the analyte concentration in the sample by virtue of a reaction sequence comprising an analyte-specific reaction taking place in the reaction layer and an electrode reaction including transfer of electrons through an electrode surface. A plurality of values of a curve of the analyte detection current versus time are measured and the analyte concentration is derived therefrom by means of an evaluation algorithm. Fast results of high accuracy are achieved in that the DC-voltage is applied to the electrodes during the analyte-specific reaction whereby the analyte-specific reaction and the electrode reaction take place generally simultaneously resulting in an analyte detection current versus time curve which comprises a rising section in which the current increases versus time and the analyte detection current is measured at at least two points of time in the rising section and the values of the analyte detection current resulting from said at least two measurements are used in the evaluation algorithm for error compensation.
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Citations
46 Claims
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1. Biosensor system for the electrochemical determination of an analyte concentration in a sample, the system comprising
an analyte reactant and at least two electrodes, wherein the analyte reactant is at least partially hydrated by the sample to form a reaction layer in contact with the electrodes for measurement of an analyte detection current flowing between the electrodes in response to an analysis voltage applied thereto, the analyte detection current corresponding to the analyte concentration in the sample by virtue of a reaction sequence comprising an analyte-specific reaction taking place in the reaction layer and an electrode reaction including transfer of electrons through a surface of one of the electrodes; - and
electronic circuitry comprising a DC-voltage source for applying the analysis voltage to the electrodes and measurement and evaluation electronics for measuring a plurality of values along a curve of the analyte detection current versus time over a plurality of measurement times and for deriving the analyte concentration therefrom by means of an evaluation algorithm; wherein the analysis voltage is applied to the electrodes during the analyte-specific reaction, the analyte-specific reaction and the electrode reaction taking place generally simultaneously and resulting in an analyte detection current versus time curve which comprises a rising section in which the current increases versus time; and wherein the analyte detection current is measured at at least two points of time in the rising section and the values of the analyte detection current resulting from said at least two measurements are used in the evaluation algorithm for interferent error compensation. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 25, 26)
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15. Method for the electrochemical determination of an analyte concentration in a sample, comprising the steps of
at least partially hydrating an analyte reactant with the sample to form a reaction layer, contacting the reaction layer to at least two electrodes, applying an analysis voltage to the electrodes, measuring a plurality of values of a curve of an analyte detection current versus time over a plurality of measurement times, the analyte detection current flowing between the electrodes in response to the analysis voltage and corresponding to the analyte concentration in the sample by virtue of a reaction sequence comprising an analyte-specific reaction taking place in the reaction liquid and an electrode reaction including transfer of electrons through a surface of one of the electrodes, and deriving the analyte concentration by means of an evaluation algorithm comprising use of said plurality of values of the analyte detection current, wherein the analysis voltage is applied to the electrodes during the analyte-specific reaction whereby the analyte-specific reaction and the electrode reaction take place generally simultaneously resulting in an analyte detection current versus time curve which comprises a rising section in which the current increases versus time; - and
wherein the analyte detection current is measured at at least two points of time in the rising section and the values of the analyte detection current resulting from said at least two measurements are used in the evaluation algorithm for interferent error compensation. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24)
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- 27. A biosensor system for the electrochemical determination of an analyte concentration in a sample comprising an analysis element that is pluggable into an evaluation instrument, the analysis element having at least two electrodes and an analyte reactant, the evaluation instrument having electronic circuitry configured to apply an analysis voltage to the electrodes of the analysis element relatively immediately after the sample is detected on the analysis element and to measure a plurality of current values over time up to at least the time at which a maximum current value is measured, and to use the plurality of values in an algorithm for interferent error compensation.
- 32. An evaluation instrument for use with an analysis element having at least two electrodes and an analyte reagent, the instrument comprising electronic circuitry configured to apply an analysis voltage to the electrodes relatively immediately after a sample is detected at the electrodes, and to measure a plurality of current values over time resulting from the application of the analysis voltage, up to at least the time at which a maximum current value is measured, and to use the plurality of values in an algorithm for interferent error compensation.
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36. A method for the electrochemical determination of an analyte concentration in a sample, the sample forming a reaction layer by at least partially hydrating an analyte reactant, comprising the steps of
applying an analysis voltage to the reaction layer, measuring over time a plurality of values of an analyte detection current resulting from the analysis voltage, identifying a maximum current value measured during the measuring step, terminating the measuring step relatively immediately after the identifying step, and deriving the analyte concentration by means of an evaluation algorithm comprising use of said plurality of values of the analyte detection current, the evaluation algorithm including compensation for interferent error.
- 43. An evaluation instrument for the electrochemical determination of an analyte concentration in a sample, the instrument comprising an electronic circuitry configured to adapt the time required for obtaining measurement results to be relative to the approximate time at which a maximum current value is measured in a reaction layer in electrical connection with the electronic circuitry, the reaction layer comprising an analyte reactant at least partially hydrated by the sample and having an analysis voltage applied thereto by the instrument relatively immediately after the reaction layer is detected by the instrument.
Specification