Immunoassay
First Claim
1. An immunoassay for detection and/or quantification of cardiac troponin I (cTnI) in a sample derived from an individual'"'"'s blood, comprisingcontacting said sample with at least two capture antibodies that bind to said cTnI and at least one tracer antibody that binds to said cTnI or to TnC complexed with cTnI,detecting a signal from said tracer antibody,comparing said signal against a signal obtained from a standard having a known concentration of cardiac troponin I,wherein a first capture antibody is directed to the N-terminal portion of cTnI, andwherein a second capture antibody is directed to the C-terminal portion of cTnI.
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Accused Products
Abstract
A method for detection and/or quantification of cardiac troponin I (cTnI) in a sample derived from an individual'"'"'s blood, the method being based on a sandwich immunoassay employing at least two capture antibodies and at least one tracer antibody, in which a first capture antibody is directed to the N-terminal part of cTnI, to the C-terminal part of cTnI or to the part of the cTnI midfragment, which is slightly affected by the interfering factor, and a second capture antibody is directed to another of these parts, and a tracer antibody is directed to the N-terminal part of cTnI, to the C-terminal part of cTnI or to TnC, which is complexed with cTnI.
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Citations
13 Claims
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1. An immunoassay for detection and/or quantification of cardiac troponin I (cTnI) in a sample derived from an individual'"'"'s blood, comprising
contacting said sample with at least two capture antibodies that bind to said cTnI and at least one tracer antibody that binds to said cTnI or to TnC complexed with cTnI, detecting a signal from said tracer antibody, comparing said signal against a signal obtained from a standard having a known concentration of cardiac troponin I, wherein a first capture antibody is directed to the N-terminal portion of cTnI, and wherein a second capture antibody is directed to the C-terminal portion of cTnI.
Specification