Fluorescent proteins and methods for using same

• US 7,638,615 B1
• Filed: 01/09/2007
• Issued: 12/29/2009
• Est. Priority Date: 01/25/2006
• Status: Active Grant

• Current Assignee: Evrogen IP Joint Stock Company
• Original Assignee: Evrogen IP Joint Stock Company
• Inventors: Chudakov, Dmitry M., Lukyanov, Sergey A.
• Primary Examiner(s): Nashed; Nashaat T
• Assistant Examiner(s): Tsay; Marsha M
• Application Number:
  US11/651,728
• Publication Number:
  US 20100015701A1
• Time in Patent Office: 1,085 Days
• Field of Search: None
• US Class Current: 536/23.1
• CPC Class Codes: C07K 14/43504 from invertebrates
• CPC Class Codes: C07K 14/43595 from coelenteratae, e.g. medusae

Abstract

The present invention provides nucleic acid molecules encoding novel red fluorescent proteins from Entacmaea quadricolor and mutants thereof. Also of interest are proteins that are substantially similar to the novel red fluorescent proteins. In addition, host cells, stable cell lines and transgenic organisms comprising the nucleic acid molecules encoding the novel red fluorescent proteins are provided. The subject proteins and nucleic acid compositions find use in a variety of different applications and methods, particularly for labeling of biomolecules, cells, or cell organelles. Finally, kits for use in such methods and applications are provided.

Claims

1. An isolated nucleic acid, comprising a nucleic acid sequence encoding a fluorescent protein, wherein said protein has at least 95% sequence identity to a fluorescent protein of SEQ ID NO:

12.

2. An isolated nucleic acid according to claim 1 wherein the fluorescent protein comprises an amino acid sequence of SEQ ID NO:

12.

3. A vector comprising the nucleic acid according to claim 1.

4. A vector comprising the nucleic acid according to claim 2.

5. An expression cassette comprising (a) a transcriptional initiation region functional in an expression host;

(b) the nucleic acid according to claim 1; and

(c) and a transcriptional termination region functional in said expression host.

6. An expression cassette comprising (a) a transcriptional initiation region functional in an expression host;

(b) the nucleic acid according to claim 2; and

(c) and a transcriptional termination region functional in said expression host.

7. A host cell or progeny thereof, comprising the expression cassette according to claim 5 as part of an extrachromosomal element or integrated into the genome of a host cell as a result of introduction of said expression cassette into said host cell.

8. A host cell or progeny thereof, comprising the expression cassette according to claim 6 as part of an extrachromosomal element or integrated into the genome of a host cell as a result of introduction of said expression cassette into said host cell.

9. A transgenic cell, or progeny thereof, comprising the nucleic acid according to claim 1.

10. A transgenic cell, or progeny thereof, comprising the nucleic acid according to claim 2.