Method of measuring blood component and sensor used in the method

• US 7,641,785 B2
• Filed: 09/28/2004
• Issued: 01/05/2010
• Est. Priority Date: 10/02/2003
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Inventors: Shinno, Teppei, Ikeda, Shin
• Primary Examiner(s): Nguyen; Nam X
• Assistant Examiner(s): Salzman; Kourtney R
• Application Number:
  US10/954,020
• Publication Number:
  US 20050145490A1
• Time in Patent Office: 1,925 Days
• Field of Search: None
• US Class Current: 205/777.5
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/3274 Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration coding of calibration information G01N33/48771
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose
• CPC Class Codes: G01N 33/721 Haemoglobin

Abstract

A sensor for blood component analysis that can correct the effect of a hematocrit easily is provided. The sensor includes an analysis portion including a working electrode, a counter electrode, and a reagent portion. The reagent portion includes an oxidoreductase that reacts with the blood component and a mediator, and the blood component is measured by causing a redox reaction between the blood component and the oxidoreductase in the presence of the mediator and detecting a redox current generated by the redox reaction by the working electrode and the counter electrode. In this sensor, the reagent portion further includes a hemolyzing agent (e.g., sodium cholate) for hemolyzing an erythrocyte, and when detecting the redox current, the erythrocyte is hemolyzed with the hemolyzing agent so as to cause hemoglobin released to an outside of the erythrocyte to react with the mediator and a current generated by this reaction also is detected to correct an effect of a hematocrit.

Claims

1. A method of measuring a blood component, comprising:

bringing a sample containing blood into contact with a reagent containing an oxidoreductase that reacts with the blood component in the blood, a mediator, and a hemolyzing agent for hemolyzing an erythrocyte;

detecting a current generated by mixing of the sample and the reagent, the current being detected by electrodes; and

converting the detected current value into an amount of the blood component,

wherein the mixing of the sample and the reagent causes a redox reaction between the blood component in the blood and the oxidoreductase, hemolysis of the erythrocyte in the blood and a reaction between hemoglobin released to an outside of the erythrocyte and the mediator,

wherein detecting the current generated by mixing detects the current value that has been corrected to remove an effect of a hematocrit, and

wherein the amount of the blood component obtained by converting the detected current value is the blood component amount that has been corrected to remove the effect of the hematocrit.

2. The method according to claim 1, wherein the hemolyzing agent is a membrane protein solubilizer.

3. The method according to claim 2, wherein the membrane protein solubilizer is a cholic acid-based detergent.

4. The method according to claim 3, wherein the cholic acid-based detergent is at least one selected from the group consisting of cholic acid, sodium cholate, cholic acid methyl ester, chenodeoxycholic acid, sodium chenodeoxycholate, diphenylglycolic acid (benzilic acid), deoxycholic acid, sodium deoxycholate, sodium glycochenodeoxycholate, glycocholic acid, sodium glycocholate, glycodeoxycholic acid, sodium glycodeoxycholate, glycolic acid, sodium glycolate, sodium glycolithocholate, lithocholic acid, sodium thioglycolate, sodium taurocholate, sodium taurodeoxycholate, sodium tauroursodeoxycholate, sodium ursodeoxycholate, and ursodeoxycholic acid.

5. The method according to claim 2, wherein an amount of the membrane protein solubilizer is 0.01 mM to 100 mM per one measurement.

6. The method according to claim 1, wherein the mediator is potassium ferricyanide.

7. The method according to claim 1, wherein the blood component as an analyte is at least one selected from the group consisting of glucose, lactic acid, uric acid, bilirubin, and cholesterol.

8. The method according to claim 1, wherein the blood component as an analyte is glucose, and the oxidoreductase is at least one of glucose oxidase and glucose dehydrogenase.

9. A sensor for measuring a blood component, comprising an analysis portion., the analysis portion comprising:

a working electrode;

a counter electrode; and

a reagent portion, the reagent portion comprising (1) an oxidoreductase that reacts with the blood components, (2) a mediator, and (3) a hemolyzing agent for hemolyzing an erythrocyte,

wherein within the analysis portion, the erythrocyte is hemolyzed with the hemolyzing agent, a redox reaction is caused between the blood component and the oxidoreductase in the presence of the mediator, a reaction is caused between hemoglobin released to an outside of an erythrocyte by the hemolysis and the mediator, and a current generated by the redox reaction and the reaction between the hemoglobin and the mediator is detected by the working electrode and the counter electrode, whereby an amount of the blood component that has been corrected to remove an effect of a hematocrit can be measured.

10. The sensor according to claim 9, wherein the hemolyzing agent is a membrane protein solubilizer.

11. The sensor according to claim 10, wherein the membrane protein solubilizer is a cholic acid-based detergent.

12. The sensor according to claim 11, wherein the cholic acid-based detergent is at least one selected from the group consisting of cholic acid), sodium cholate, cholic acid methyl ester, chenodeoxycholic acid, sodium chenodeoxycholate, diphenylglycolic acid (benzilic acid), deoxycholic acid, sodium deoxycholate, sodium glycochenodeoxycholate, glycocholic acid, sodium glycocholate, glycodeoxycholic acid, sodium glycodeoxycholate, glycolic acid, sodium glycolate, sodium glycolithocholate, lithocholic acid, sodium thioglycolate, sodium taurocholate, sodium taurodeoxycholate, sodium tauroursodeoxycholate, sodium ursodeoxycholate, and ursodeoxycholic acid.

13. The sensor according to claim 10, wherein an amount of the membrane protein solubilizer is 0.01 mM to 100 mM per one sensor.

14. The sensor according to claim 9, wherein the mediator is potassium ferricyanide.

15. The sensor according to claim 9, wherein the blood component as an analyte is at least one selected from the group consisting of glucose, lactic acid, uric acid, bilirubin, and cholesterol.

16. The sensor according to claim 9, wherein the blood component as an analyte is glucose, and the oxidoreductase is at least one of glucose oxidase and glucose dehydrogenase.

17. The sensor according to claim 9, wherein the reagent portion further comprises a hydrophilic polymer, an enzyme stabilizer, and a crystal homogenizing agent.

18. The sensor according to claim 9, further comprising an insulating substrate, wherein the working electrode and the counter electrode are disposed on the insuiating substrate, thereby forming the analysis portion, the reagent portion is disposed on the analysis portion, the analysis portion communicates with one end of a channel for leading blood to the analysis portion, and the other end of the channel is open toward an outside of the sensor, thereby allowing this opening to serve as a blood supply port.

19. The sensor according to claim 18, further comprising a spacer and a cover, wherein the cover is disposed on the insulating substrate with the spacer intervening between the cover and the insulating substrate.

20. The sensor according to claim 9, further comprising a detecting electrode, wherein the detecting electrode is located farther from the blood inlet port than the analysis portion, and whether or not blood is supplied to the analysis portion is detected by the detecting electrode.

21. A method of measuring a blood component, comprising:

hemolyzing an erythrocyte in blood so as to cause hemoglobin to be released to an outside of the erythrocyte;

causing a redox reaction between the blood component and an oxidoreductase in the presence of a mediator so as to cause the hemoglobin released to the outside of the erythrocyte to react with the mediator;

detecting a current generated by the redox reaction and the reaction between the hemoglobin and the mediator by electrodes, and

converting the detected current value into an amount of the blood component, whereby the blood component amount that has been corrected to remove an effect of a hematocrit is measured.