Labeled probe bound object, method for producing the same and method for using the same

• US 7,642,086 B2
• Filed: 08/07/2006
• Issued: 01/05/2010
• Est. Priority Date: 08/09/2005
• Status: Expired due to Fees

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Okamoto, Tadashi
• Primary Examiner(s): Lam; Ann Y
• Application Number:
  US11/499,711
• Publication Number:
  US 20070092897A1
• Time in Patent Office: 1,247 Days
• Field of Search: None
• US Class Current: 435/287.2
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: C12Q 2563/137 Metal/ion, e.g. metal label
• CPC Class Codes: C12Q 2563/155 Particles of a defined size, e.g. nanoparticles
• CPC Class Codes: C12Q 2563/185 Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals

Abstract

A method for labeling an object, which is effective when multiple types of labels are given as necessary even to a very small object such as a fine particulate substrate for use in fixation of biological substances such as probe molecules or nucleic acid primers that are used for a bioassay is provided. Further, an object given the labels is provided. Multiple types of labels can be given as necessary even to very small objects by using materials for labeling prepared in accordance with the composition condition of contained atoms corresponding to at least binary system n-digit numerical value information using a plurality of types (n types) of atoms selected beforehand as part of materials constituting the object, and presence/absence of the selected individual atoms contained or the level of the contents thereof. Further, the bioassay can be performed by the object labeled by the labeling method.

Claims

1. A set of plural particles used for discrimination of a target substance, comprising at least 31 particles,

wherein each of the plural particles binds on its surface to a biological substance,

wherein each of the plural particles comprises a label corresponding to the bound biological substance, and

wherein the plural particles are labeled by at least 31 types of labels discriminated by the existence/non-existence of at least 5 types of atoms.

2. The set of plural particles according to claim 1, wherein the biological substance is a probe.

3. The set of plural particles according to claim 2, wherein the probe is one of a pair of substances selected from nucleic acids, DNAs, RNAs, cDNAs, cRNAs, oligodeoxyribonucleotides, polydeoxyribonucleotides, oligoribonucleotides, polyribonucleotides, peptide nucleic acids (PNAs), oligopeptide nucleic acids, peptides, oligopeptides, polypeptides, proteins, antigens, antibodies, enzymes, ligands, ligand receptors, sugars and sugar chains and capable of being bound with the substances recognizing each other.

4. The set of plural particles according to claim 1, wherein the biological substance is a nucleic acid primer.

5. The set of plural particles according to claim 4, wherein the nucleic acid primer is selected from the group consisting of oligodeoxyribonucleotides, polydeoxyribonucleotides, oligoribonucleotides and polyribonucleotides.

6. The set of plural particles according to claim 5, wherein the nucleic acid primer is bound covalently to the surface of the particle directly or indirectly via a linker at the 5′

terminal side of the primer.

7. The set of plural particles according to claim 1, wherein at least one of the plural particles is a magnetic material.

8. The set of plural particles according to claim 1, wherein the at least 5 types of atoms are selected from the group consisting of Ni, Mn, Zn, Cu, Mg, Al and Ti.

9. The set of plural particles according to claim 1, wherein the label is identified by at least one of mass spectrometry, X-ray photoelectron spectroscopy and Auger electron spectroscopy.

10. The set of plural particles according to claim 1, wherein the label is included in the particle.