×

Methods for monitoring drug activities in vivo

  • US 7,643,943 B2
  • Filed: 03/05/2004
  • Issued: 01/05/2010
  • Est. Priority Date: 02/11/2003
  • Status: Expired due to Fees
First Claim
Patent Images

1. A method for monitoring CCI-779 activity in a patient having a non-blood disease, the method comprising:

  • (a) generating an expression profile of an ubiquitin specific protease 11 (USP11) gene in a peripheral blood sample obtained from the patient having the non-blood disease and at a stage of treatment with CCI-779, wherein the expression profile is generated by isolating a plurality of ribonucleic acid (RNA) molecules from the peripheral blood sample, labeling said plurality of RNA molecules with a labeling moiety, and probing said plurality of RNA molecules for the presence of a USP11 RNA;

    (b) comparing the expression profile of said USP11 gene generated from step (a) to a reference expression profile of said USP11 gene; and

    (c) making a determination of CCI-779 activity in the patient based on the comparison result from step (b), wherein(i) the determination is provided to a user,(ii) said USP11 gene is differentially expressed in peripheral blood mononuclear cells (PBMCs) of patients who have the non-blood disease during the treatment with CCI-779 as compared to PBMCs of said patients before the treatment, and(iii) the non-blood disease is a solid tumor.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×