Identification of compounds for inhibiting complexation of C-reactive protein with fibronectin
First Claim
1. A method for identifying a gibberellic acid or a jasmonic acid compound that inhibits the complexation of C-reactive protein with fibronectin, the method comprising:
- contacting a first polypeptide with a second polypeptide in the presence of a gibberellic acid or jasmonic acid test compound to form a complex of the first polypeptide with the second polypeptide, the first polypeptide comprising at least a segment of a C-reactive protein, the first polypeptide including the fibronectin binding site of the C-reactive protein and the second polypeptide comprising at least a segment of a fibronectin protein, the second polypeptide including the C-reactive binding site of the fibronectin; and
determining whether complexation of the first polypeptide with the second polypeptide is decreased in the presence of said gibberellic acid or jasmonic acid test compound as compared to a control containing no gibberellic acid or jasmonic acid compound, wherein the first polypeptide and the second polypeptide form a complex in the absence of gibberellic acid or jasmonic acid compound and a decrease in said complexation is an indication that said gibberellic acid or jasmonic acid compound inhibits the complexation of C-reactive protein to fibronectin.
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Accused Products
Abstract
Disclosed are screening processes for identification of compounds that may inhibit complex formation between C-reactive protein and fibronectin. Compounds identified by the disclosed methods may be utilized to inhibit the target analytes that are known to occur during skin aging as well as during the course of several diseases. Accordingly, inhibitory compounds identified by the disclosed methods may be utilized to prevent and treat damaged tissue, inflammatory conditions, cardiovascular conditions, renal conditions, periodontal conditions and obesity, among other conditions. For example, the compounds identified by the disclosed methods may be utilized to treat tissue that has suffered trauma, e.g., burns or wounds, as well as tissue that is inflamed due to any of several causes. The disclosed compounds may also be utilized to improve the accuracy of assays designed to assess CRP or Fn levels in a sample, for instance in a plasma sample, as a risk assessment tool in certain medical conditions and to discover new treatments.
45 Citations
17 Claims
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1. A method for identifying a gibberellic acid or a jasmonic acid compound that inhibits the complexation of C-reactive protein with fibronectin, the method comprising:
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contacting a first polypeptide with a second polypeptide in the presence of a gibberellic acid or jasmonic acid test compound to form a complex of the first polypeptide with the second polypeptide, the first polypeptide comprising at least a segment of a C-reactive protein, the first polypeptide including the fibronectin binding site of the C-reactive protein and the second polypeptide comprising at least a segment of a fibronectin protein, the second polypeptide including the C-reactive binding site of the fibronectin; and determining whether complexation of the first polypeptide with the second polypeptide is decreased in the presence of said gibberellic acid or jasmonic acid test compound as compared to a control containing no gibberellic acid or jasmonic acid compound, wherein the first polypeptide and the second polypeptide form a complex in the absence of gibberellic acid or jasmonic acid compound and a decrease in said complexation is an indication that said gibberellic acid or jasmonic acid compound inhibits the complexation of C-reactive protein to fibronectin. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification