Kit and method for multi-analyte determination
First Claim
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1. A method for the simultaneous qualitative and/or quantitative determination of a multitude of analytes comprising:
- bringing one or more liquid samples to be analyzed for the analytes into contact with measurement areas of a sensor platform,the sensor platform comprisingan optical thin film waveguide with a first layer, that is transparent at least at one excitation wavelength, on a second layer that has a lower refractive index than the first layer and is also transparent at least at the excitation wavelength, and at least one grating structure modulated in the first layer for in-coupling of the excitation light into the first layer;
at least one array of biological or biochemical or synthetic recognition elements immobilized in measurement areas for detection of different analytes by specific recognition and/or binding of said analytes and/or by specific interaction with said analytes,means for laterally resolved referencing of the excitation light intensity available in the measurement areas and, optionally,means for the calibration of one or more luminescences generated in a near field of the first layer,the measurement areas for detection of different analytes being usable for detection of analytes to be determined and/or as means for calibration purposes;
irradiating an excitation light towards the measurement areas;
detecting an emission light from the measurement areas;
referencing the excitation light intensity available in the measurement areas in a locally resolved way by means of correcting the emission light intensity from measurement areas for detection of different analytes using the means for laterally resolved referencing of the excitation light intensity available in the measurement areas;
calibrating one or more luminescences generated in a near-field of the first layer from the measurement areas that result from the binding of one or more analytes to biological or biochemical or synthetic recognition elements immobilized in the measurement areas or from interaction between the analytes and the immobilized recognition elements using the measurement areas for detection of different analytes as means for calibration purposes to establish a calibration curve on each platform; and
comparing the emission light intensity from measurement areas for detection of analytes in the sample to be analyzed to the calibration curve for qualitative and/or quantitative determination of the analyte.
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Abstract
The invention is related to different embodiments of a kit for the simultaneous qualitative and/or quantitative determination of a multitude of analytes comprising
- a sensor platform comprising an optical thin-film waveguide with a layer (a) transparent at least at an excitation wavelength on a layer (b) with lower refractive index than layer (a), also transparent at least at said excitation wavelength, and at least one grating structure (c) modulated in said layer (a), for the incoupling of said excitation light into layer (a),
- at least one array of biological or biochemical or synthetic recognition elements immobilized in discrete measurement areas (d) directly or by means of an adhesion-promoting layer on layer (a), for specific recognition and/or binding of said analytes and/or for specific interaction with said analytes,
- means for laterally resolved referencing of the excitation light intensity available in the measurement areas, and optionally
- means for the calibration of one or more luminescences generated in the near-field of layer (a) as a consequence of the binding of one or more analytes or of the specific interaction with one or more analytes,
wherein a liquid sample to be analyzed for said analytes is brought into contact with said measurement areas on said sensor platform either directly or after mixture with further reagents. The invention is also related to analytical systems based on a kit according to the invention and to methods for the determination of one or more analytes, based on said kit, and to use thereof.
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Citations
30 Claims
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1. A method for the simultaneous qualitative and/or quantitative determination of a multitude of analytes comprising:
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bringing one or more liquid samples to be analyzed for the analytes into contact with measurement areas of a sensor platform, the sensor platform comprising an optical thin film waveguide with a first layer, that is transparent at least at one excitation wavelength, on a second layer that has a lower refractive index than the first layer and is also transparent at least at the excitation wavelength, and at least one grating structure modulated in the first layer for in-coupling of the excitation light into the first layer; at least one array of biological or biochemical or synthetic recognition elements immobilized in measurement areas for detection of different analytes by specific recognition and/or binding of said analytes and/or by specific interaction with said analytes, means for laterally resolved referencing of the excitation light intensity available in the measurement areas and, optionally, means for the calibration of one or more luminescences generated in a near field of the first layer, the measurement areas for detection of different analytes being usable for detection of analytes to be determined and/or as means for calibration purposes; irradiating an excitation light towards the measurement areas; detecting an emission light from the measurement areas; referencing the excitation light intensity available in the measurement areas in a locally resolved way by means of correcting the emission light intensity from measurement areas for detection of different analytes using the means for laterally resolved referencing of the excitation light intensity available in the measurement areas; calibrating one or more luminescences generated in a near-field of the first layer from the measurement areas that result from the binding of one or more analytes to biological or biochemical or synthetic recognition elements immobilized in the measurement areas or from interaction between the analytes and the immobilized recognition elements using the measurement areas for detection of different analytes as means for calibration purposes to establish a calibration curve on each platform; and comparing the emission light intensity from measurement areas for detection of analytes in the sample to be analyzed to the calibration curve for qualitative and/or quantitative determination of the analyte. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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Specification