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Methods of producing and sequencing modified polynucleotides

  • US 7,645,866 B2
  • Filed: 06/28/2006
  • Issued: 01/12/2010
  • Est. Priority Date: 06/28/2005
  • Status: Expired due to Fees
First Claim
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1. A method for producing a modified polynucleotide sequence comprising:

  • a) annealing at least one primer to a template polynucleotide sequence;

    b) extending said primer in the presence one or more nucleoside triphosphates,wherein at least one of the nucleoside triphosphates is a modified nucleoside triphosphate comprising a general structure [I];

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