Fluorescent phantom device

• US 7,649,185 B2
• Filed: 03/06/2007
• Issued: 01/19/2010
• Est. Priority Date: 02/06/2002
• Status: Expired due to Term

• Current Assignee: Xenogen Corporation (Revvity, Inc.)
• Original Assignee: Xenogen Corporation (Revvity, Inc.)
• Inventors: Nilson, David G., Troy, Tamara L., Rice, Bradley W.
• Primary Examiner(s): Porta; David P
• Assistant Examiner(s): Boosalis; Faye
• Application Number:
  US11/682,710
• Publication Number:
  US 20070200058A1
• Time in Patent Office: 1,050 Days
• Field of Search: 250/458.1, 250/459.1, 250/252.1, 600/476, 600/478
• US Class Current: 250/458.1
• CPC Class Codes: A61B 2560/0233 Optical standards
• CPC Class Codes: A61B 5/0071 by measuring fluorescence emission
• CPC Class Codes: A61B 5/0073 by tomography, i.e. reconstruction of 3D images from 2D projections A61B5/0066 takes precedence
• CPC Class Codes: A61B 5/0084 for introduction into the body, e.g. by catheters A61B5/1459 takes precedence
• CPC Class Codes: G01J 1/08 Arrangements of light sources specially adapted for photometry standard sources, also using luminescent or radioactive material
• CPC Class Codes: G01N 21/278 Constitution of standards
• CPC Class Codes: G01N 21/4795 spatially resolved investigating of object in scattering medium in vivo A61B
• CPC Class Codes: G01N 21/643 non-biological material
• CPC Class Codes: G09B 23/30 Anatomical models G09B23/281 - G09B23/288 take precedence

Abstract

Described herein is a phantom device that simplifies usage, testing, and development of light imaging systems. The phantom device includes a body and a fluorescent light source internal to the body. The body comprises an optical material designed to at least partially resemble the optical behavior of mammalian tissue. The phantom device has many uses. One use of the phantom device permits testing of tomography software in the imaging system, such as software configured for 3D reconstruction of the fluorescent light source. Another use tests spectral unmixing software in the imaging system. The phantom device also allows a user to compare trans- and epi-fluorescent illumination imaging results.

Claims

1. A phantom device comprising:

a body including one or more surfaces and an optical material designed to at least partially resemble the optical behavior of mammalian tissue; and

a fluorescent light source disposed within the body and configured to emit fluorescent light from within the body, through the optical material and to the one or more surfaces.

2. The device of claim 1 wherein the fluorescent light source is stored in a cavity internal to the body.

3. The device of claim 2 further comprising a hole in the body sized to receive a removable member, wherein the cavity is formed in the removable member.

4. The device of claim 3 wherein the removable member has a cylindrical shape that includes the cavity.

5. The device of claim 2 wherein the removable member includes optical material that matches the optical material used in the body.

6. The device of claim 2 further comprising a second hole in the body sized to receive the removable member.

7. The device of claim 6 wherein the second hole has a different vertical position or a different lateral position than the first hole when the phantom device rests on a flat surface.

8. The device of claim 6 further comprising a second removable member configured to fit within the second hole, where the second removable member includes the optical material.

9. The device of claim 1 wherein the fluorescent light source includes a fluorophore.

10. The device of claim 9 wherein the fluorophore is suitable for use as a biological reporter in a mammal.

11. The device of claim 1 wherein the optical material is configured to resemble an autofluorescence spectrum of mammalian tissue.

12. The device of claim 1 wherein the fluorescent light source includes between about 10¹⁰ to about 10¹⁶ molecules of a fluorophore.

13. The device of claim 1 wherein surface topography of the body is designed to match the surface topography of a mammal.

14. The device of claim 1 wherein the optical material is substantially homogeneous.

15. The device of claim 1 wherein the optical material is configured to resemble an optical absorption property or optical scattering property of mammalian tissue.

16. A phantom device comprising:

a body including one or more surfaces and an optical material designed to at least partially resemble the optical behavior of mammalian tissue;

a hole in the body;

a removable member including a cavity and shaped to fit in the hole; and

a fluorescent light source disposed in the cavity and configured to emit fluorescent light from within the body when the removable member is in the hole.

17. The device of claim 16 wherein the removable member has a cylindrical shape that includes the cavity.

18. The device of claim 17 wherein the removable member includes optical material that matches the optical material used in the body.

19. The device of claim 16 further comprising a second hole in the body sized to receive the removable member.

20. The device of claim 19 wherein the second hole has a different height or a different lateral position than the first hole.

21. The device of claim 19 further comprising a second removable member configured to fit within the second hole, where the second removable member includes the optical material and does not include a cavity.

22. A method for testing a light imaging system, the method comprising:

receiving a phantom device, the phantom device comprising a body including an optical material designed to at least partially resemble the optical behavior of mammalian tissue and a fluorescent light source internal to the body;

illuminating the body with excitation light that causes the fluorescent light source to emit fluorescent light;

capturing an image of at least a portion of a phantom device;

building a digital representation of the fluorescent light source using data included in the image; and

comparing a component of the digital representation to a known property for the fluorescent light source or the phantom device.

23. The method of claim 22 further comprising spectrally unmixing the image to produce a) a digital representation of the fluorescent light source and b) a digital representation of autofluorescence from the body.

24. The method of claim 23 wherein the optical material is configured to resemble an autofluorescence of mammalian tissue.

25. The method of claim 22 wherein the incident light is provided from below the body.

26. The method of claim 22 wherein the incident light is provided from above the body.

27. The method of claim 22 further comprising illuminating the body with second excitation light that causes the fluorescent light source to emit fluorescent light, wherein the first excitation light is provided from below the body and the second excitation light is provided from above the body.

28. The method of claim 27 further comprising:

capturing a second image of at least the portion of a phantom device;

building a second digital representation of the fluorescent light source using data included in the second image; and

comparing a component of the first digital representation to a component of the second digital representation for the fluorescent light source or the phantom device.

29. The method of claim 22 further comprising assessing performance of the light imaging system using the comparison.

30. The method of claim 22 further comprising calibrating the light imaging system using the comparison.

31. The method of claim 30 wherein the fluorescent light source includes between about 10¹⁰ to about 10¹⁶ molecules of a fluorophore.

32. The method of claim 22 wherein the known property for the light source comprises one of:

intensity for the light source, a size of the light source, and a spectral pattern for the light source.

33. The method of claim 22 wherein the known property for the phantom device comprises one of:

a surface size for a surface of the phantom device, a location of the light source within the phantom device, and a spectral response for the optical material.

34. The method of claim 22 wherein the component comprises one of:

a flux from a surface of the phantom device, an estimated intensity of the light source, an estimated location of the light source within the phantom device, and an estimated size of the light source.

35. A computer readable medium including instructions for testing a light imaging system, the instructions comprising:

instructions for capturing an image of at least a portion of a phantom device, said phantom device having a body including one or more surfaces and an optical material designed to at least partially resemble the optical behavior of mammalian tissue, and also a fluorescent light source disposed within the body and configured to emit fluorescent light from within the body, through the optical material and to the one or more surfaces;

instructions for building a digital representation of the fluorescent light source using data included in the image; and

instructions for comparing a component of the digital representation to a known property for the fluorescent light source or the phantom device.