Polymerizable emulsions for tissue engineering
First Claim
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1. A method of preparing a nontoxic, viscoelastic material for tissue engineering, wherein the viscoelastic material comprises a polymerized emulsion having two discrete organic and aqueous phases, the method comprising:
- a) preparing a nontoxic, inverse (“
water-in-oil”
) fluid emulsion comprising two discrete phases;
i) a continuous hydrophobic organic (“
oil”
) phase, comprising one or more reactive, ethylenically unsaturated, polymerizable acrylate monomers, and at least one activator- controlled polymerization initiator; and
ii) a discontinuous aqueous (“
water”
) phase comprising water,wherein the aqueous phase is present in the emulsion in an amount of about 0.5% to about 90% weight/weight of the organic phase; and
b) polymerizing the fluid emulsion in situ to form the viscoelastic material, wherein the viscoelastic material comprises;
i) a continuous hydrophobic organic phase comprised of polymerized ethylenically unsaturated acrylate monomers;
ii) a discontinuous aqueous (“
water”
) phase comprising water.
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Abstract
Provided are biocompatible viscoelastic solid materials derived from polymerization of fluid water-in-oil emulsions, along with methods of their preparation and methods for their use for tissue engineering applications, including for reforming diseased, damaged or degenerated intervertebral discs by acceptably non-invasive means.
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Citations
32 Claims
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1. A method of preparing a nontoxic, viscoelastic material for tissue engineering, wherein the viscoelastic material comprises a polymerized emulsion having two discrete organic and aqueous phases, the method comprising:
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a) preparing a nontoxic, inverse (“
water-in-oil”
) fluid emulsion comprising two discrete phases;i) a continuous hydrophobic organic (“
oil”
) phase, comprising one or more reactive, ethylenically unsaturated, polymerizable acrylate monomers, and at least one activator- controlled polymerization initiator; andii) a discontinuous aqueous (“
water”
) phase comprising water,wherein the aqueous phase is present in the emulsion in an amount of about 0.5% to about 90% weight/weight of the organic phase; and b) polymerizing the fluid emulsion in situ to form the viscoelastic material, wherein the viscoelastic material comprises; i) a continuous hydrophobic organic phase comprised of polymerized ethylenically unsaturated acrylate monomers; ii) a discontinuous aqueous (“
water”
) phase comprising water. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 32)
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28. A method for preparing a nontoxic, viscoelastic material for tissue engineering, wherein the viscoelastic material comprises a polymerized emulsion having two discrete organic and aqueous phases, the method comprising:
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a) preparing a nontoxic, inverse (“
water-in-oil”
) fluid emulsion comprising two discrete phases;
i) a continuous hydrophobic organic (“
oil”
) phase, comprising one or more reactive, ethylenically unsaturated, polymerizable acrylate monomers, and a peroxide free radical generator; and
ii) a discontinuous aqueous (“
water”
) phase, wherein the aqueous phase is present in an amount of about 0.5% to about 90% weight/weight of the organic phase;b) preparing a nontoxic, inverse (“
water-in-oil”
) fluid emulsion comprising two discrete phases;
i) a continuous hydrophobic organic (“
oil”
) phase, comprising one or more reactive, ethylenically unsaturated, polymerizable acrylate monomers, and an amine catalyst; and
ii) a discontinuous aqueous (“
water”
) phase, wherein the aqueous phase is present in an amount of about 0.5% to about 90% weight/weight of the organic phase; andc) combining the emulsions of steps a) and b) to initiate polymerization to form the viscoelastic material comprising of; i) a continuous hydrophobic organic phase comprised of polymerized ethylenically unsaturated acrylate monomers; and ii) a discontinuous aqueous (“
water”
) phase comprising water. - View Dependent Claims (29, 30, 31)
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Specification