Methods of using adipose tissue-derived cells in augmenting autologous fat transfer
First Claim
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1. A method of processing adipose tissue to augment autologous fat transfer to a subject, comprising:
- removing a first portion of unprocessed adipose tissue that comprises a cell population that comprises adipose-derived stem cells from a subject;
introducing the first portion of unprocessed adipose tissue that comprises said cell population that comprises adipose-derived stem cells into a self-contained adipose-derived stem cell processing unit, wherein said self-contained adipose derived stem cell processing unit comprises;
a tissue collection chamber that is configured to receive unprocessed adipose tissue that is removed from a patient, wherein said tissue collection chamber is defined by a closed system;
a first filter that is disposed within said tissue collection chamber, wherein said first filter is configured to retain a first component of said unprocessed adipose tissue and pass a second component of said unprocessed adipose tissue, such that said first filter separates said first component from said second component, and wherein said first component comprises a cell population that comprises adipose-derived stem cells and said second component comprises lipid, mature adipocytes, and saline;
a processing chamber, which is configured to receive said first component comprising said cell population that comprises adipose-derived stem cells from said tissue collection chamber, wherein said processing chamber is within said closed system;
a conduit configured to allow passage of said first component comprising said cell population comprising adipose-derived stem cells from said tissue collection chamber to said processing chamber while maintaining a closed system;
a cell concentrator disposed within said processing chamber, which is configured to facilitate the concentration of said first component comprising said cell population that comprises adipose-derived stem cells so as to obtain a concentrated population of cells that comprises adipose-derived stem cells, wherein said cell concentrator comprises a centrifuge or a filter; and
an outlet configured to allow the aseptic removal of said concentrated population of cells that comprise adipose-derived stem cells;
separating and concentrating said cell population that comprises adipose-derived stem cells from said first portion of adipose tissue within said self-contained adipose-derived stem cell processing unit while maintaining said closed pathway to obtain a concentrated cell population that comprises adipose-derived stem cells;
mixing said concentrated cell population that comprises adipose-derived stem cells with a second portion of unprocessed adipose tissue comprising intact, non disaggregated tissue fragments from said subject so a to obtain a mixture of the unprocessed adipose tissue and the concentrated cell population that comprises adipose-derived stem cells; and
providing said mixture of the unprocessed adipose tissue and the concentrated cell population that comprises adipose-derived stem cells to said subject.
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Abstract
Methods of treating patients for conditions such as breast augmentation, soft tissue defects, and urinary incontinence, are described. The methods include removing adipose tissue from a patient, processing a portion of the adipose tissue to obtain a substantially isolated population of regenerative cells, mixing the regenerative cells with another portion of adipose tissue to form a composition, and administering the composition to the patient from which the adipose tissue was removed.
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Citations
23 Claims
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1. A method of processing adipose tissue to augment autologous fat transfer to a subject, comprising:
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removing a first portion of unprocessed adipose tissue that comprises a cell population that comprises adipose-derived stem cells from a subject; introducing the first portion of unprocessed adipose tissue that comprises said cell population that comprises adipose-derived stem cells into a self-contained adipose-derived stem cell processing unit, wherein said self-contained adipose derived stem cell processing unit comprises; a tissue collection chamber that is configured to receive unprocessed adipose tissue that is removed from a patient, wherein said tissue collection chamber is defined by a closed system; a first filter that is disposed within said tissue collection chamber, wherein said first filter is configured to retain a first component of said unprocessed adipose tissue and pass a second component of said unprocessed adipose tissue, such that said first filter separates said first component from said second component, and wherein said first component comprises a cell population that comprises adipose-derived stem cells and said second component comprises lipid, mature adipocytes, and saline; a processing chamber, which is configured to receive said first component comprising said cell population that comprises adipose-derived stem cells from said tissue collection chamber, wherein said processing chamber is within said closed system; a conduit configured to allow passage of said first component comprising said cell population comprising adipose-derived stem cells from said tissue collection chamber to said processing chamber while maintaining a closed system; a cell concentrator disposed within said processing chamber, which is configured to facilitate the concentration of said first component comprising said cell population that comprises adipose-derived stem cells so as to obtain a concentrated population of cells that comprises adipose-derived stem cells, wherein said cell concentrator comprises a centrifuge or a filter; and an outlet configured to allow the aseptic removal of said concentrated population of cells that comprise adipose-derived stem cells; separating and concentrating said cell population that comprises adipose-derived stem cells from said first portion of adipose tissue within said self-contained adipose-derived stem cell processing unit while maintaining said closed pathway to obtain a concentrated cell population that comprises adipose-derived stem cells; mixing said concentrated cell population that comprises adipose-derived stem cells with a second portion of unprocessed adipose tissue comprising intact, non disaggregated tissue fragments from said subject so a to obtain a mixture of the unprocessed adipose tissue and the concentrated cell population that comprises adipose-derived stem cells; and providing said mixture of the unprocessed adipose tissue and the concentrated cell population that comprises adipose-derived stem cells to said subject. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification