Coating solution for glucose sensing membrane and method of manufacturing optical glucose sensor chip
First Claim
1. A method of manufacturing an optical glucose sensor chip having a glucose sensing membrane containing an oxidizing enzyme or a reducing enzyme of glucose, a reagent for generating a substance for coloring a coloring agent, a coloring agent, and a membrane-forming polymer compound, comprising:
- preparing a first solution by dissolving the oxidizing enzyme or the reducing enzyme of glucose and a reagent for generating a substance for coloring a coloring agent in a buffer solution and then preserving the first solution;
preparing a second solution by mixing an alcohol solution of the coloring agent and a solution of the membrane-forming polymer compound and then preserving the second solution;
forming a pair of gratings on a glass substrate for receiving and sending light in the substrate;
preparing a coating solution for the glucose sensing membrane by mixing the first solution and the second solution before the coating solution is applied to a substrate region among said gratings; and
forming the glucose sensing membrane by applying the coating solution to the substrate region among the gratings and drying the solution;
wherein the substrate region among the gratings is subjected to dry washing with excimer ultraviolet rays and wet treatment with an acid solution, before the coating solution is applied to the substrate region among the gratings.
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Accused Products
Abstract
A method of preparing a coating solution for a glucose sensing membrane, the method comprising preserving a first solution containing an oxidizing enzyme or a reducing enzyme of glucose and a reagent for generating a substance for coloring a coloring agent dissolved in a buffer solution, preserving a second solution containing a mixed solution of an alcohol solution of the coloring agent and a solution of a membrane-forming polymer compound, and mixing the first solution and the second solution.
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Citations
16 Claims
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1. A method of manufacturing an optical glucose sensor chip having a glucose sensing membrane containing an oxidizing enzyme or a reducing enzyme of glucose, a reagent for generating a substance for coloring a coloring agent, a coloring agent, and a membrane-forming polymer compound, comprising:
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preparing a first solution by dissolving the oxidizing enzyme or the reducing enzyme of glucose and a reagent for generating a substance for coloring a coloring agent in a buffer solution and then preserving the first solution; preparing a second solution by mixing an alcohol solution of the coloring agent and a solution of the membrane-forming polymer compound and then preserving the second solution; forming a pair of gratings on a glass substrate for receiving and sending light in the substrate; preparing a coating solution for the glucose sensing membrane by mixing the first solution and the second solution before the coating solution is applied to a substrate region among said gratings; and forming the glucose sensing membrane by applying the coating solution to the substrate region among the gratings and drying the solution; wherein the substrate region among the gratings is subjected to dry washing with excimer ultraviolet rays and wet treatment with an acid solution, before the coating solution is applied to the substrate region among the gratings. - View Dependent Claims (2)
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3. A method of manufacturing an optical glucose sensor chip having a glucose sensing membrane containing an oxidizing enzyme or a reducing enzyme of glucose, a reagent for generating a substance for coloring a coloring agent, a coloring agent, and a membrane-forming polymer compound comprising:
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preparing a first solution by dissolving the oxidizing enzyme or the reducing enzyme of glucose and a reagent for generating a substance for coloring a coloring agent in a buffer solution and then preserving the first solution; preparing a second solution by mixing an alcohol solution of the coloring agent and a solution of the membrane-forming polymer compound and then preserving the second solution; forming a pair of gratings on a glass substrate for receiving and sending light in the substrate; preparing a coating solution for the glucose sensing membrane by mixing the first solution and the second solution before the coating solution is applied to the substrate region among the gratings; and forming the glucose sensing membrane by applying the coating solution to the substrate region among the gratings and drying the solution, wherein the first solution is preserved at a temperature lower than the melting point of the solvent in the first solution and the second solution is preserved at a temperature of 4°
C. to 10°
C. - View Dependent Claims (4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method of manufacturing an optical glucose sensor chip, comprising:
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forming a pair of gratings on a glass substrate for receiving and sending light in the substrate; and forming a glucose sensing membrane by applying a coating solution to a substrate region among the gratings and drying the solution; wherein the substrate region among the gratings is subjected to dry washing with excimer ultraviolet rays and wet treatment with an acid solution before the coating solution is applied to the substrate region among the gratings; and wherein the coating solution is produced at the time of use by mixing; a first solution comprising a solvent, a glucose oxidizing enzyme or a glucose reducing enzyme, a reagent for generating a substance for coloring a coloring agent, and a buffer that prevents decomposition of the glucose oxidizing or reducing enzyme, which first solution has been stored at a temperature lower than the melting point of the solvent, with a second alcohol solution containing a coloring agent and a membrane-forming polymer compound. - View Dependent Claims (16)
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Specification