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Compositions and kits for detection of a target nucleic acid

  • US 7,655,405 B2
  • Filed: 11/29/2006
  • Issued: 02/02/2010
  • Est. Priority Date: 10/29/1999
  • Status: Expired due to Fees
First Claim
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1. A kit for generating a signal indicative of the presence of a target nucleic acid sequence in a sample, comprising a thermostable Fen nuclease consisting of a 5′

  • to 3′

    exonuclease and/or endonuclease activity, a nucleic acid polymerase that substantially lacks 5′

    to 3′

    exonuclease activity, a nucleic acid primer, and a labeled nucleic acid complementary to said target nucleic acid sequence.

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