Compositions and kits for detection of a target nucleic acid
First Claim
Patent Images
1. A kit for generating a signal indicative of the presence of a target nucleic acid sequence in a sample, comprising a thermostable Fen nuclease consisting of a 5′
- to 3′
exonuclease and/or endonuclease activity, a nucleic acid polymerase that substantially lacks 5′
to 3′
exonuclease activity, a nucleic acid primer, and a labeled nucleic acid complementary to said target nucleic acid sequence.
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Abstract
The present invention is directed to kits and compositions for generating a signal indicative of the presence of a target nucleic acid sequence in a sample, comprising a nucleic acid polymerase lacking 5′ to 3′ exonuclease activity and a thermostable FEN nuclease consisting of a 5′ to 3′ exonuclease and/or endonuclease activity.
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Citations
32 Claims
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1. A kit for generating a signal indicative of the presence of a target nucleic acid sequence in a sample, comprising a thermostable Fen nuclease consisting of a 5′
- to 3′
exonuclease and/or endonuclease activity, a nucleic acid polymerase that substantially lacks 5′
to 3′
exonuclease activity, a nucleic acid primer, and a labeled nucleic acid complementary to said target nucleic acid sequence. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 22, 23, 24, 28, 30, 31, 32)
- to 3′
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11. A composition comprising an isolated thermostable nucleic acid polymerase substantially lacking 5′
- to 3′
exonuclease activity, a isolated thermostable Fen nuclease consisting of a 5′
to 3′
exonuclease and/or endonuclease activity, and a nucleic acid primer. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 25, 26, 27, 29)
- to 3′
Specification