Cyanine dye compounds

• US 7,655,409 B2
• Filed: 09/29/2008
• Issued: 02/02/2010
• Est. Priority Date: 12/05/2003
• Status: Active Grant

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Huang, Shih-Jung, Yue, Stephen, Dallwig, Jason, Thomas, Gerald, Hagen, David
• Primary Examiner(s): Riley; Jezia
• Application Number:
  US12/240,218
• Publication Number:
  US 20090047683A1
• Time in Patent Office: 491 Days
• Field of Search: 435/6, 536/23.1, 536/24.3, 536/24.33, 536/26.6
• US Class Current: 435/6.12
• CPC Class Codes: C09B 23/04 one >CH- group, e.g. cyanines, isocyanines, pseudocyanines
• CPC Class Codes: C12Q 1/6827 for detection of mutation or polymorphism
• CPC Class Codes: C12Q 2527/125 Specific component of sample, medium or buffer
• CPC Class Codes: C12Q 2563/107 fluorescence
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

Cyanine dye compounds having a negatively charged substituent that are nucleic acid stains, particularly for fluorescent staining of DNA, including compounds having the formula

wherein W forms one or two fused 5- or 6-membered aromatic rings, α has a value of 0 or 1, n has a value of 0, or 1, X is O, S, or Se, and D is a pyridinium, or quinolinium moiety, provided that the compound has at least one negatively charged substituent.

Claims

1. A method for detecting DNA in the presence of RNA, wherein the method comprises the steps:

a. combining a nucleic acid reporter molecule with a sample to prepare a labeling mixture, wherein the nucleic acid reporter molecule has a DNA/RNA ratio of fluorescence enhancement greater than about one wherein the nucleic acid reporter molecule is according to the formula

2. The method according to claim 1, further comprising quantifying the DNA present in the sample.

3. The method according to claim 1, wherein the sample comprises biological fluids, buffer solutions, live cells, fixed cells, eukaryotic cells, prokaryotic cells, nucleic acid polymers, nucleotides, nucleosides, a polymeric gel or tissue sections.

4. The method according to claim 1, wherein the sample comprises cells, tissue, or biological fluids.

5. The method according to claim 1, wherein the sample is present in or on a microarray or a microwell plate.