Bioinformatically detectable group of novel regulatory oligonucleotides and uses thereof

• US 7,655,785 B1
• Filed: 04/02/2004
• Issued: 02/02/2010
• Est. Priority Date: 11/14/2002
• Status: Active Grant

• Current Assignee: Rosetta Genomics Limited (Rosetta Genomics)
• Original Assignee: Rosetta Genomics Limited (Rosetta Genomics)
• Inventors: Bentwich, Itzhak
• Primary Examiner(s): McGarry; Sean R
• Assistant Examiner(s): MCDONALD, JENNIFER SUE PITRAK
• Application Number:
  US10/708,953
• Time in Patent Office: 2,132 Days
• Field of Search: None
• US Class Current: 536/24.1
• CPC Class Codes: C12N 15/113 Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing when used in plants C12N15/8218
• CPC Class Codes: C12N 2310/141 MicroRNAs, miRNAs
• CPC Class Codes: C12Q 1/6886 for cancer immunoassay for cancer G01N33/574
• CPC Class Codes: C12Q 2600/178 miRNA, siRNA or ncRNA

Abstract

The present invention relates to a first group of novel oligonucleotides, here identified as Genomic Address Messenger or GAM oligonucleotide, and a second group of novel operon-like polynucleotides, here identified as Genomic Record or GR polynucleotide. GAM oligonucleotides selectively inhibit translation of known ‘target’ genes, many of which are known to be involved in various diseases. Nucleic acid molecules are provided respectively encoding 15454 GAM precursors oligonucleotides, and 681 GR polynucleotides, as are vectors and probes both comprising the nucleic acid molecules, and methods and systems for detecting GAM oligonucleotides and GR polynucleotides and specific functions and utilities thereof, for detecting expression of GAM oligonucleotides and GR polynucleotides, and for selectively enhancing and selectively inhibiting translation of the respective target genes thereof.

Claims

1. An isolated nucleic acid consisting of X nucleotides wherein the sequence of the nucleic acid comprises:

(a) Y consecutive nucleotides of SEQ ID NO;

2240728;

(b) a DNA equivalent of (a);

or

(c) the complement of (a) or (b),

wherein,

X=16 to 120,

Y≧

16, and

X≧

Y.

2. The nucleic acid of claim 1, wherein the Y nucleotides comprises the sequence of SEQ ID NO:

8385.

3. The nucleic acid of claim 1, wherein X=19 to 24 nucleotides.

4. The nucleic acid of claim 1, wherein X=Y.

5. The nucleic acid of claim 4, wherein the Y consecutive nucleotides is SEQ ID NO:

2240728.

6. The nucleic acid of claim 4, wherein the Y consecutive nucleotides is SEQ ID NO:

8385.

7. A vector comprising a human insert, wherein the human insert consists of the nucleic acid of claim 1, and wherein the vector comprises no other insert but the nucleic acid of claim 1.

8. A vector comprising a human insert, wherein the human insert consists of the nucleic acid of claim 2, and wherein the vector comprises no other insert but the nucleic acid of claim 2.

9. A vector comprising a human insert, wherein the human insert consists of the nucleic acid of claim 3, and wherein the vector comprises no other insert but the nucleic acid of claim 3.

10. A vector comprising a human insert, wherein the human insert consists of the nucleic acid of claim 4, and wherein the vector comprises no other insert but the nucleic acid of claim 4.

11. A vector comprising a human insert, wherein the human insert consists of the nucleic acid of claim 5, and wherein the vector comprises no other insert but the nucleic acid of claim 5.

12. A vector comprising a human insert, wherein the human insert consists of the nucleic acid of claim 6, and wherein the vector comprises no other insert but the nucleic acid of claim 6.

13. The isolated nucleic acid of claim 1, wherein the nucleic acid is a probe.

14. The isolated nucleic acid of claim 2, wherein the nucleic acid is a probe.

15. The isolated nucleic acid of claim 3, wherein the nucleic acid is a probe.

16. The isolated nucleic acid of claim 4, wherein the nucleic acid is a probe.

17. The isolated nucleic acid of claim 5, wherein the nucleic acid is a probe.

18. The isolated nucleic acid of claim 6, wherein the nucleic acid is a probe.